Hypertension Membrane Ion Transport And Its Influence Factors

The etiology and pathogenesis of hypertension has been catching much of the attention. In recent years, studies on these subjects are gradually deepened at the cellular or molecular level. It is thought that the important points in pathogenesis of hypertension may be the abnormalities in cellular contents of electrolytes, in permeability of cell membrane to Na⁺ and Ca²⁺ in transportation of cation. In this study, to further characterize these abnormalities we simultaneously studied the role of the activity of Na⁺ pump and Ca²⁺ pump on cell membrane and the changes in cellular electrolytes played in the pathogenesis of hypertension. The regulatory factors of Na⁺ pump and Ca²⁺ pump and cation transportation through cell membrane and the correlation of these factors with other biologically active substances were further investigated. The study includs animal and clinical experiments.â…  . Animal experiments were performed with spontaneously hypertensive rats (SHR) to investigate:(1) The changes in activity of Na⁺ pump and Ca²⁺ pump of erythrocytes, myocardium and vascular tissue.(2) The changes in contents of K⁺, Na⁺, Ca²⁺ and Mg²⁺ in erythrocytes, myocardium and vascular tissue.(3) The relationship between blood pressure and the changes of free Ca²⁺ in platelet and Ca²⁺ intake by erythrocytes inside—out vesicle (IOV).(4) The regulatory or influential factors to Na⁺ and Ca²⁺ pump and intracellular contents of electrolytes, including: â‘  Vascular-pressor substances: plasma renin activity (PRA), angiotensin â…¡ (AT â…¡ ), norepinephrine (NE) and epinephrine (E), â‘¡ the second messenger CAMP, Calmodulin (CaM) and â‘¢ endogenous digitalis—liks factor (EDLF) and plasma electrolytes.â…¡. Clinical experiments include patients of essential hypertension, secondary hypertension, borderline hypertension and normotensive offspring who had a family history of hypertension. The drug interfering experiments were performed in these patients to observe:(1) The changes in activity of Na⁺ and Ca²⁺ pump of erythrocytes and the changes in contents of K⁺, Na⁺, Ca²⁺ and Mg²⁺ in erythrocytes.(2) The relationship between hypertension and free Ca²⁺ in platelet and in plasma, and the capability of Ca²⁺ intaking by IOV.