| Background: Atherosclerosis (AS) is a disease which is characterized by many theories.Major riskfactors of AS are associated with abnormal blood lipid levels,hypertension,cigarettesmoking,diabetes.In the past few years, the mortality rate for AS has markedly and progressivelyincreased in China. AS also causes a great deal of serious illness which included coronary arterydisease (CAD), cerebrovascular disease.This article reviewed the advancement of traditionalChinese and modem medicine about AS.Objective: To investigate the mechanism of changed chinese herbal medicine Da-Chai-Hu-Tang ininhibiting AS in rabbits, observing the effects of Da-Chai-Hu-Tang for the formation of AS,theexpression of phenotype of smooth muscle cell of vessel wall and c-myc, monocyte chemoattractantprotein-1 (MCP-1),CD40,intercellular adhesion molecule (ICAM-1) in AS.Methods: The models of AS were set up by feeding containing hyperlipemia.The rabbits weredivided randomly into five groups:normal control group,AS model group,Da-Chai-Hu-Tangprevention group and cure group,xuezhikang positive control group. Da-Chai-Hu-Tang cure groupwas administered at the 6th week after models was set up,the other groups were feeded for 10weeks, observing the effect of Da-Chai-Hu-Tang for blood lipid,oxygen free radical,cAMP reactiveprotein. After sacrificing the animals, differential parts were obsevered, viewing aorticpathomorphology by light microscope and electron microscope and observing cellular phenotypeson smooth muscle cell and researching the effect for the phaenotype of smooth muscle cell of vesselwall and the expression of c-myc by hybridization in situ, studing MCP-1,CD40,ICAM-1,mRNAand expression of protein by reverse transcriptase PCR (RT-PCR),Westem Blot andimmunohistochemistry.Results: The levels of total cholesterol(TC),triglyeride(TG),low density lipoproteincholesterol(LDL-C) in serum were significantly lower and the activity of SOD,phospholipidhydroperoxide glutathione peroxidase (PHGPx) was improved in Da-Chai-Hu-Tang group.Thedegree of CRP was lowered Da-Chai-Hu-Tang prevention group has an advantage overDa-Chai-Hu-Tang cure group and xuezhikang positive control group.There was significantdifference (P＜0.05,P＜0.01).The expression of MCP-1 and ICAM-1 was less in Da-Chai-Hu-Tangprevention group than Da-Chai-Hu-Tang cure group and xuezhikang positive control group(P＜0.01).For the effect of the expression of CD40 and CL40L, there was significant difference betweenDa-Chai-Hu-Tang prevention group and cure group(P＜0.01), while there was no significantdifference between Da-Chai-Hu-Tang prevention group and xuezhikang positive control group.Conclusion: The prevention and cure mechanism of Da-Chai-Hu-Tang might be concerned withadjusting blood lipid,preventing the process of lipid peroxidation, inhibiting the phenotypic changeof smooth muscle cell and the expression of c-myc and inflammatory factor.This study providedthe evidence of theroy for the application of Da-Chai-Hu-Tang.The relation of phlegmatic stagnation and inflammatory factor was explained in study oftheory. Discussing hyperlipemia from phlegmatic stagnation,the prevention and cure ofhyperlipemia ,the study of health cultivation of Chinese medical science, CAD from phlegmatic,the relation of CD40 and AS,monocyte chemotactic factor and AS,the advancement of research for ASin modem medicine, the overview of Chinese medicine for AS,which were included in the article.Itwas effective in discussing AS from phlegmatic stagnation, investigating the name of illness,causeof disease,differentiation of symptoms and signs, treatment, some problems in study.it was indicatedfurther that the character of pathology, pathogenesy and therapeutic principle of AS, investigatingthe effect of inflammatory factor, CD40 and MCP-1 in AS.
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