Studies On Chemome And Metabolome Of Gardenia Jasminoides Ellis By Multiple-dimensional Chromatography-electrospray Ionization Mass Spectrometry

The systematic and comprehensive study on active components from Gardeniajasminoides Ellis. was carried out. Ten iridoid glycosides were obtained bymultiple-dimensional chromatography, and the preparation protocol of the active componentsgroup had also been established. Moreover, the rapid isolation platforms of some compoundsby on-line HPLC-HPLC column switch technique and on-line purity monitoringHSCCC-HPLC-DAD system had been established. Chemical fingerprint and metabolicfingerprint analysis of Gardenia jasminoides Ellis. by HPLC-DAD-ESI-MS technique wasestablished and the preclinical pharmacokinetics of the iridoid glycosides was also studied.