| Objective:To study the multiple chemical components in vitro(iridoid,chlorogenic acid,two terpenes pigment) content changes in fried pieces used in Gardenia(Gardenia clinical students,fried gardenia, Jiao Zhizi) the initial establishment of ratsserum fingerprints of Gardenia Jasminoids, clear changes in composition and content ofeffective substances in the serum of Fructus Gardeniae pieces, in direct material and group composition and its variation, in-depth interpretation of Fructus Gardeniae pieces the pharmacodynamic material basis and the principle of processing, which laid the foundation for the further research of Fructus Gardeniae pieces.Methods:(1) In Geniposidic as an internal matter, the use of QAMS to study the simultaneous determination of different frying slices of Gardenia detected at 254 nm wavelength nine iridoid glycosides and chlorogenic acid, the chemical composition of a total of two types of high-performance liquid chromatography(2) Using UV- spectrophotometry, respectively Geniposidic, crocin as standard, frying slices of Gardenia different total iridoid glycosides, total pigment content was measured.(3) Infrared spectroscopy gardenia frying slices of different qualitative analysis(4) tdynamic serum chemistry methods, serum samples at different time points were analyzed using LC-MS in rat serum after oral administration of normal chemical constituents were detected. Using LC-MS/MS for the simultaneous determination of methyl deacetyl aspsrulosidate,GEGE and geniposidecontent, comparison of gardenia sub-frying process, three kinds of composition differences in pharmacokinetics in ratsResults : QAMS method by a correction factor of nine components were: 0.5171,0.6955, 0.9045,0.9281,0.72981.9843,0.9358,1.2865,0.3874. Determination results show: the total amount of iridoid glycosides ring and chlorogenic acid raw gardenia, coke gardenia, Gardenia carbon nine showed a decline trend. UV spectrophotometric study showed that total iridoid glycosides in 5.248-36.736 ug /ml range a good linear relationship, the total pigment in 1.0128-6.0768ug/ml good linear relationship between the two methodological precision, repeat, stability, average recoveries were 0.11-0.59% RSD%. IR Qualitative Study: Health and gardenia, coke gardenia, Gardenia charcoal one-dimensional map of the peak shape is similar, but in the second derivative spectra of the 1228 cm-1,1241 cm-1 and 1636 in the two-dimensional atlas(1640) cm-1,1740cm-1 band is quite different. The use of LC-MS, was detected in serum absorbed gardenia four prototype components: Jasminoidin acid, deacetylation car asiatica leaf methyl GEGE double gentian, Geniposidic. Kinetic studies on the pharmacokinetics of the three components of gardenia rats serum Show: MEDA. GEGE GENIP of linear ranges were: 0.1018-3.2576 ug /ml, 0.0761-9.7440 ug /ml, 0.0750-9.600ug/ml, the relative recoveries were 85%-115%, day and inter-day precision and stability of the RSD% were less than 10%, to meet the requirements of biological sample measured, indicating that this method is accurate, good stability, can be accurately measured. Health Gardenia bioavailability of three components is generally higher than gardenia charcoal, coke Gardenia lowest bioavailability, as concocted a deeper level, the focus gardenia, Gardenia carbon residence time was significantly longer in the bodyConclusion: QAMS can be used for quantitative analysis of Gardenia Pieces, content analysis of 10 components, preliminary interpretation of the gardenia frying process variation of 10 chemical components; to UV spectrophotometric determination of total iridoid Gardenia terpene glycosides, total pigment can be used for quality control of gardenia; using infrared spectroscopy to identify the different frying slices of Gardenia characteristic fingerprint and compare three different peaks can be used to identify different frying slices of Gardenia. LC-MS techniques can Gardenia attribution components into the blood serum, using a triple quadrupole LC quantitative analysis, research deacetylase car methyl gardenia leaves asiatica different slices serum GEGE double glucoside, the dynamic changes of geniposidic. This paper from the determination, qualitative identification, serum chemistry, pharmacokinetics, etc. multi-component, multi-angle dynamic changes of gardenia elaborated during frying modern basic connotation of substances in vivo, in order to clarify the gardenia concocted principles provide a scientific basis. |
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