| Ficus microcarpa Linn.f.belongs to the genus Ficus of family Moraceae, which is also called Xiaoyerong,Xiyerong,Wannianqing,etc.It's aerial root and leaves both can be used as medicine,the former called as Rongxu and the latter called as Xiaoyerong.It is widely distributed in the tropical and subtropical areas in China.Due to the effects of clearing heat,removing damp,relieving cough and eliminating phlegm,promoting blood circulation to remove blood stasis, Xiaoyerong has been widely used for the treatment of bronchitis,influenza, pertussis,tonsillitis,bacterial dysentery,enteritis,conjunctival congestion, toothache,traumatic injury,etc.But Xiaoyerong has not been adopted by the Chinese pharmacopea and it's chemical constituents have been scarcely reported. So in this research,systematically studies on it's chemical constituents and quality control were carried out,and antitussive,expectorant and anti-imflammatory effects were investigated.So as to provide theoretical proof and practical reference for its clinical application and further research.1.Extraction and SeparationIn this thesis,studies on the chemical constituents of Xiaoyerong,17 compounds had been isolated from the dried folium of Ficus microcarpa Linn.f. Based on their physicochemical and spectroscopic evidence,17 structures were elucidated as follows:3 flavonoids:vitexin,orientin,isovitexin;8 triterpenoids and sterols:β-amyrone,β-sitosterol,lupeol,lupeopl acetate,β-amyrin,β-amyrin acetate,daucosterol,maslinic acid;6 alcohol,acid and esters:stearic acid, pentadecyl acid,n-Octacosanol,n-Octacosanol,hexacosanoic acid.Among these compounds,exceptβ-sitosterol,daucosterol,epifriedelinol,maslinic acid,the other compounds were isolated for the first time from this plant;and vitexin, orientin and isovitexin were isolated for the first time from genus Ficus.2.Volatile oil Components AnalysisVolatile oil in Xiaoyerong was also analyzed,29 compounds had been identificated.The main constituents of this oil were terpenoids and their oxides, which accounts for 95.13 percent of the total volatile oil.Phytol and patchouli alcohol were the higher contents.3.Study on the Pharmacological EffectsThree methods had been used in experiments,which were investigated the effects of antitussive,apophlegmatic and anti-inflammatory effects of Xiaoyerong. The results showed that water extract and ethyl acetate extract had obviously antitussive apophlegmatic and anti-inflammatory activities,and the latter's effect was stronger than the former.So ethyl acetate extract is the effective fraction of Xiaoyerong,and flavonoid is effective components of Xiaoyerong4.Study on Quality StandardsIn this thesis,studies on the quality control of Xiaoyerong were performed, including characteristics,microconstruction and physical and chemical character, quantitative determination of total flavonoid by UV-VIS,vitexin by HPLC. Through the investigation of variations of effective components,optimum collecting time and harvest periods were established.And based on those,a quality standard of Xiaoyerong was drafted to provide reference for clinical practice.In summary,in this thesis,through the systematical studies mentioned above on Xiaoyerong,part of its chemical constituents has been determinde.Its characteristics,microconstruction,physical and chemical character,quantitative determination and pharmacological effects have been studied.These provided the theoretical basis and scientific reference for further study,development and application of Ficus microcarpa Linn.f.
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