The Experimental Study Of Somatostatin Combined With Angiostatin Gene Inhibiting The Growth Of Human Pancreatic Cancer

Objective To investigate the effect of somastatin combined with angiostatin genc to inhibit human pancreatic cancinoma cell proliferation and to induce apoptosis in vitro and in vivo.Methods The pcDNA3/angio was transfected into human BXPC-3 pancreatic cancer cell by liposome-mediated gene transfer method.RT-PCR and Western blot were used to detect the expression of angiostatin gene.Method:In vitro,MIT and flow cytometry(FCM)were used to detect whether somastatin combined with angiostatin can effect inhibit the growth of BXPC-3 and ECV-304 cells.Xengraft human pancreatic cancer models were established through BXPC-3 cells subcutaneously,in nude mice They received injection of pcDNA3,pcDNA3/angio, somastatin more injection into the tumor.We observed the growth of tumor and measured the volume of xenografts.The VEGF and MVD expression were detected by immunohistochemistry.The apoptosis of pancreatic cancer cell more detected by transmission electron microscope.Result A 700bp specific base pair was obtained by RT-PCR.The expression of angiostatin protein was confirmed by Western blot.In vitro,the result of MIT and FCM showed that somastatin(≥10ug/ml)can inhibit the growth of BXPC-3 and induce apoptosis.But it can't inhibit the growth of ECV-304.It was confirmed that angiostatin can inhibit the growth of ECV-304 and induce apoptosis by MTI,FCM detection.But angiostatin can't inhibit the growth of BXPC -3.In vivo,somastatin and angiostatin can inhibit the growth of human pancreatic cancer.They can inhibit the production of new blood vessel in human pancreatic cancer.They decrease the expression of VEGF and induce the apoptosis of human pancreatic cancer cell. Conclusion:(1).Somastain can directly inhibit the proliferation of human pancreatic cancer cells and induce apoptosis.They can't directly inhibit angiogenesiso of human pancreatic cancer.(2).Angiostatin can specially inhibit the proliferation of ECV-304 and induce apoptosis.It can inhibit angiogenesis of human pancreatic cancer. (3).Somastatin combined with angiostatin gene have coe-synergistic effect of anti-angiogenesis and inhibiting the proliferation of human pancreatic cancer.