The Intervention Of Decorin On Leptomeninge's Fibrosis Due To SAH

Objective:Subarachnoid hemorrhage(SAH)is a common disease to neurology and neurosurgery,and chronic hydrocephalus is a common chronic complication after SAH.Leptomeninges include aracnoid mater, pia mater and subarachnoid space,arachnoid cell is a kind of specific fibroblast.TGF-β₁ can modulate positively fibroblast functions of proliferation and secretion.After SAH,the elevation of TGF-ββ₁ level in cerebrospinal fluid can lead to fibrosis of leptomeninges(aracnoid, subarachnoid space)and arachnoid villi,which result in chronic hydrocephalus. Decorin(DCN)is generally referred as a natural antagonist of TGF-β₁ and have effect of anti-fibrosis.To a certain degree,it has realistic significance to choose DCN as precaution against chronic hydrocephalus in cases with SAH because the fibrosis of leptomeninges(aracnoid,subarachnoid space)and arachnoid villi might be diminished by DCN.Up to now,there is no reports on this field.The purpose of this study is to preliminary research the intervention of DCN on leptomeninges' fibrosis due to SAH.Methods:First,80 SD rats were randomly devided into blank group, normal salineâ… group(NSâ… group)and modelâ… group(SAHâ… group),to build up the animal models of SAH by twice injecting autoblood solution into the cisterna magna.At the 3^rd,6^th,10^th,14^th,21^stday,rats were respectively killed,the brain of the 3^rdwere observed to detect the blood clot in subarachnoid space,and the levels of TGF-β1 and PICP in collected CSF of all were measured to detect their dynamic variation,and lepomeninges of the 21^stday's were measured to detect the fibrotic degree.Then,40 SD rats were randomly devided into experimental group, control group,SAHâ…¡group and NSâ…¡group.Decorin was injected into the cisterna magna of experimental group and control group at the 2^nd,10^TH.At the 21^stday,we measured the levels of TGF-β1 and PICP in CSF, and the fibrotic degree of lepomeninges.The methods of measuring the fibrotic degree include collagen fiber Masson staining,RT-PCR measuring mRNA of Collagenâ… (Colâ… )and CTGF,Western-blot to measuring protein of Colâ… and CTGF.Results:1.Construction of animal model of SAIl:â‘ At the 3^rd, scattered blood clot could be observed in subarachnoid space and cisterna; Red blood cells could be observed in the space of leptomeninges between dura mater and brain tissue on microslides by Masson staining;â‘¡The levels of TGF-β1 in CSF of SAHâ… group rose statistically by double phase,compared with control group(P＜0.01);â‘¢The levels of PICP in CSF of SAHâ… group were gradually increasing and obviously higher than that of NSâ… group after the 6^th(P＜0.01);â‘£Masson staining showed that the gray scale of lepomenings in SAHâ… group was obviously lower than that of NSâ… group,and thickness was obviously higher(P＜0.01);⑤The expressed level of mRNA of Colâ… ,CTGF and protein of Colâ… ,CTGF were obviously higher than that of NSâ… group(P＜0.05);â‘¥There were no signicant difference between blank group and NSâ… group on these detected items(P＞0.05).2.Intervention of DCN:â‘ Experimental group VS SAHâ…¡group: the level of TGF-β1 in experimental group was a little declining but no signicant differences(P＞0.05);the level of PICP was obviously decreasing in experimental group(P＜0.05);Masson staining showed that thickness of lepomenings in experimental group was a little thinning but no signicant differences(P＞0.05),while gray scale in experimental group was obviously increasing(P＜0.05);the expressed level of Colâ… mRNA was obviously decreasing(P＜0.05),while expressed the level of CTGF mRNA was a little decreasing but no signicant differences(P＞0.05);the protein expressed level of Colâ… and CTGF were obviously decreasing (P＜0.05).â‘¡Experimental group VS NSâ…¡group:the levels of TGF-β₁ and PICP in experimental group were obviosly increasing(P＜0.05); Masson staining showed that thickness of lepomenings in experimental group was obviously enlarging and gray scale was obviously decreasing (P＜0.05);the expressed level of mRNA of Colâ… ,CTGF and protein of Colâ… ,CTGF in experimental group were obviously increasing(P＜0.05);â‘¢Control group VS NSâ…¡group:no signicant difference on these detected items(P＞0.05).Conclusion:1.The levels of TGF-β1 in CSF was increasing by double phase after SAH;2.At the 21^stday,There were leptomeninges' fibrosis SAH;3.After the accident of SAH,DCN relieved leptomeninges' fibrosis.