The Fibrosis And The Expression Of CTGF On Leptomeningeal After Subarachnoid Hemorrhage (SAH) In Rats

Objective: Observe leptomeningeal fibrosis and the expression of connective tissue growth factor(CTGF) after subarachnoid hemorrhage(SAH) via animal models of SAH in rats.Methods: Rats were used to construct animal models of SAH by twice injecting autoblood solution into the cisterna magna with the aid of a surgical microscope on the stereotaxic frame. 21 days later, the animals were anesthetized and poured with 4% Paraform. Thereafter, their brains were removed and frozen quickly by isopentane- liquid nitrogen, and then preserved in 80℃below zero. Using multimedia chromatic colour analytical system, we did quantitative analysis towards the collagen fiber of leptomeningeal, following the frozen section, Masson and immunohistochemistry staining. The Leptomeninges fibrosis and the expression of CTGF on arachnoid cells were tested by immunohistochemistry.Results: 1. The achievement ratio of the animal models was 78.95%; moreover, leptomeninges structure was preserved well. 2. Displayed by the Masson staining, the film thickness and the staining depth of leptomeninges in experimental group increased predominantly, compared with normal sodium group(p<0.01) . There was no significant difference between normal sodium group and the blank group (p>0.01).3. The result of the test immunohistochemistry of CTGF showed that the staining depth of leptomeningeal arachnoid endochylema and duramatral fibrocyte endochylema in experimental group was increased significantly , compared with normal sodium group(p<0.01) , while no significant difference between normal sodium group and blank group was observed (p>0.01) .Conclusion: 1. It was a feasible method for studying leptomeninges fibrosis after SAH to inject twice autoblood into the cisterna magna of rats, followed by the frozen section and Masson staining. 2. Leptomeninges fibrosis was significant after SAH. 3. The expression of CTGF on arachnoid cells after SAH was increased significantly , which means CTGF may play an important role in the process of leptomeningeal fibrosis after SAH in rats.