The Empirical Study On Mechanism Of Action Of Curing Rheumatoid Arthritis With The Compatibility Composition About Radix Aconiti Preparata And Raidix Paeoniae Alba

Objective:To research the compatibility of composition about Radix Aconiti preparata and Raidix Paeoniae Alba on anti-inflammation effect,analgesia effect, deintoxication and curing rheumatoid arthritis.Find the best compatibility composition and proportion of synergistic effect.Explore the mechanism of action on compatibility composition curing rheumatoid arthritis.Methods:The pain test induced by hot-plate,the body-twisting test induced by acetic acid,the swelling test of the auricles induced by Xylene,the swelling degree test of feet induced by egg white and cold and dampness syndrome rheumatoid arthritis model of were used to research the compatibility effect of the water decoction and composition about Radix Aconiti preparata and Raidix Paeoniae Alba on anti-inflammation and analgesia and curing rheumatoid arthritis,and the acute toxicity test were used to research deintoxication.We found the best compatibility composition and proportion of synergistic effect,detoxication and curing rheumatoid arthritis.Finally,observed these indices such as endogenous opioid peptides(L-ENK,β-END) of hypothalamus,substance P,IgG of blood plasma,cell factors(IL-1β,TNF-a,IL-6,IL-2,IL-10) of blood plasma and the ultramicrostructure of synovial cell, we have explored the mechanism of action on compatibility composition curing rheumatoid arthritis.Results:1.The compatibility water decoction about Radix Aconiti preparata and Raidix Paeoniae Alba could elevate the pain threshold of mouse induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by Xylene. The compatibility water decoction about Radix Aconiti preparata and Raidix Paeoniae Alba could elevate the pain threshold,and restrain the swelling and pathological changes of rats' left or right articulus of rheumatoid arthritis of cold and dampness syndrome.The effect of most of the compatibility water decoction was better than single water decoction.The best compatibility proportion was 1:2.2.The compatibility composition about Radix Aconiti preparata and Raidix Paeoniae Alba could elevate the pain threshold of mouse induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by Xylene or the rats feet induced by egg white.The compatibility composition between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony had better analgesia effect and anti-inflammation effect.The best compatibility proportion was 1:2.The compatibility composition between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony could elevate the pain threshold,and restrain the swelling,pathological changes of rats' left or right articulus, Atrophy of thymus gland,tumidity of spleen of rheumatoid arthritis of cold and dampness syndrome.The effect of 1:2 was better than single composition.3.The LD₅₀ of All compatibility proportion between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony became more than total base of Radix Aconiti preparata,therefore,total glucosides of paeony or polysaccharides of paeony could diminish the toxicity of total base of Radix Aconiti preparata.4.The compatibility composition between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony could elevate the contents of hypothalamic L-ENK andβ-END,degrade the contents of plasmatic SP,elevate the contents of serumal IL-1βand IL-6,degrade the contents of serumal IL-2 and IL-10.The effect of 1:2 was better than single composition.Conclusions:The compatibility water decoction about Radix Aconiti preparata and Raidix Paeoniae Alba had obviously the effect of analgesia,anti-inflammation,and curing rheumatoid arthritis.The best compatibility proportion was 1:2.The compatibility composition about Radix Aconiti preparata and Raidix Paeoniae Alba had obviously the effect of analgesia,anti-inflammation,and curing rheumatoid arthritis.The best compatibility compositions were between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony.The best compatibility proportion was 1:2.The toxicity of compatibility composition between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony decreased.The compatibility composition between total base of Radix Aconiti preparata with total glucosides of paeony or polysaccharides of paeony could cure rheumatoid arthritis.The mechanism of action might be that the contents of center EOP had increased,the synthesis and release of SP had been restrained,the disturbance of serumal cell factor had been adjusted,the synthesis and secretion of serum immune globulin had been restrained,and the anomal-excitatory secretory action of synovial cell had been relieved.