Based On Evaluation Of The Effectiveness Of Multi-spectral Chromatography And Mulberry Leaf Quality Of Mulberry Leaves Mulberry,

In Pharmacopoeia of the People's Republic of China(Edition 2005),both mulberry leaf(Folium Moil) and mulberry fruit(Fructus Mori) have exclusive original plant, Morus alba Linn.Literatures indicated pharmaceutical mulberries were manifold in all ages.Medicinal materials of mulberry in markets are also sourcing from many kinds of plants of Moracease family.Mulberry leaf and mulberry fruit,having different therapeutic range,are two traditional Chinese medicines rooting from different organs of mulberries. Differentiate as well as commonness between both was shown through following experiments:immunoregulatory,relieving cough and expelling phlegm,effect on insulin receptor and antioxidant activity in vitro.Spectrum and chromatogram prints of plant medicines,which embody species distinguishability and individual comparability,reflect chemical ingredients in whole. We obtained some mulberry leaves and mulberry fruits in different breeds,the two medicines were compared severally in 5 polarity fractions,they were also contrasted with each other.Infrared spectrum(IR),thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) were used to establish unitary chemical token figures of mulberry leaf and mulberry fruit respectively.Afterwards, ultraviolet spectrophotometry(UV),TLC,HPLC were applied to draw comparisons among disparate polarity fractions of each medicine.Comparisons also occurred between two medicines' extract in same polarity fraction.Results illuminated single spectrum or chromatogram method was deficient to estimate holistic quality of traditional Chinese drug.While being divided into several parts according to polarity, the medicinal materials provided more information by means of studying the character of every polar portion.Similarities of samples were gained in all methods except TLC.Cluster analysis based on all similarities of every sample represented the likeness more logically. Ancient medicament literatures pointed out that mulberry leaf should be collected in winter for assuring the quality.HPLC was used to research mulberry leaf samples with different gather time and dissimilar breed.From the results,we discovered mulberry leaves gathered in spring have more ingredient sorts and quantity than that gathered in other seasons,yet leaves collected in winter had larger peak area in certain component.Area of total peaks,chlorogenic acid peak and four quercitin glycosides peaks were all in dynamic changes.Area of peaks above-mentioned increased from October and reached maximum on November or December,then descended rapidly.Thus it can be seen that merchandise of mulberry leaf gathered in late autumn were reassuring.Similarities of HPLC prints were relative high among Winter Folium Mori samples with different breed from same agriculture area,while lower among that with same breed but different producing place,which reflected that the environment was significant for ingredients accumulation of mulberry leaf.