Preliminary Study On The Construction Of Bioartificial Renal Tubule Assist Device In Vitro

BackgroundBioartificial renal tubule assist device(RAD) is an efficient therapy for end-stage renal disease.With the progress in study on engineering kidney,there are several problems to be solved,such as how to obtain a bioartificial kidney and how to make seeding cells growing on the surface of the scaffold?Materials and methods1.The nano-sized gold colloid was prepared by chemical reduction.Polysulfone hollow fiber membrane/nano-sized gold colloid scaffold was fabricated by self-assembly technology.Africa green monkey kidney cell line CV₁ cells were cultured on the surface of the scaffold.Inoculation time of the membrane and nano-sized gold colloid was 0h,2h,5h and 14h respectively.Cell attachment and proliferation were examined with scanning electron microscopy.2.The construction of RAD.Rat kidney proximal renal tubule cell line NRK-52E cells were seeded onto the outer space of the bioreactor hollow fiber cartridges and incubated for two weeks.And then,the transport rates of sodium and glucose were measured.Meanwhile,the inhibitory effects of oubaine and phlorizin on the transport of sodium and glucose were determined and compared with control device.Results1.Biological features of the polysulfone hollow fiber membrane/nano-sized gold colloid scaffold:Surface roughness(Ra values) of four kinds of scaffolds(0 h,2h,5h, 14h) were 29.43±12.38nm,53.02±23.52 nm,61.57±16.08 nm and 51.42±12.22 nm respectively.Water absorption ratios of them were 40.71±2.26%,61.96±3.85%, 70.26±4.24%and 63.21±4.67%respectively,and the highest ratio was seen at 5 hours.2.Growth of seeding cells on the outer surface of the polysulfone hollow fiber membranes of RAD:Cell attachment and proliferation on the 2h,5h 14h scaffolds were better than that of the Oh scaffold(P＜0.05),and the highest cell number was also seen at 5 hours.In the scaffolds(2h,5h,and 14h) groups,growth of seeded cells with many microvillus was seen on the hollow fibre surface but not the hollow fibre in control group.Transport functions of the RAD:After 14 days of incubation,cells on the RAD had functions of transporting sodium and glucose,which could be inhibited significantly by oubaine and phlorizin,and could be recovered after agents were removed.ConclusionsA bioartificial renal tubule assist device was successfully made with the polysulfone hollow fiber membrane/nano-sized gold colloid scaffold.It had increased water absorption,cell adhesion and proliferation,and had the function of transporting sodium and glucose.