Gene Expression Profiles Study On The Cerebral Gene Change Of The Model Mouse With Kidney-yang Deficiency Caused By Excessive Physical And Sexual Activities And Its Syndrome Differentiated After Using Drugs Of Therapeutic Efficacy

Objective: To inquire into the cerebral gene change of the model mouse with kidney-yang deficiency caused by excessive physical and sexual activities and its variation tested by means of the gene microarry technology, its improved syndrome change due to using drugs of efficacy such as Jinkuishenqiwan and Youguiwan which reinforcing the kidney-yang according to traditional Chinese medicine, the mechanism of the medicine, and a comparison of the genetic change between the two medicine on a molecular level.Methods: There were four groups randomly: the control group which has 10 male mice, the model group which has 15 male mice, the Jinkuishenqiwan treatment group which has 10 male mice and the Youguiwan group which has 10 male mice. All of them were fed normally, and perfused with 0.5 mL of distill water for each mouse in the control group and model group, and with 0.5 mL suspension of the drugs for each in the treatment groups every day. The experimental mice in the model group and the treatment groups were kept with six female mice each in the same cage, and all of the male mice swam until they submerged and scooped up from water everyday, which lasted four weeks. The mice might induce the kidney-yang deficiency because of excessive physical and sexual activities. The brain gene of the mice between control group, model group and treatment groups was detected with a mouse brain gene chip of 36K Mouse genome array made by CapitalBio Corp. Beijing, China, which screened the differentially expressed genes according to the ratio equal to or above 2 and equal to or below 0.5 with the related fluorescent intensity comparing with the two groups', which could be further classified in the light of gene function using Molecule Annotation System (MAS) created by CapitalBio Corp. Beijing, China, and with related reference material.Results: The animal model of kidney-yang deficiency in the model group was induced by way of excessive physical and sexual activities successfully, and the scatter plots of expressed genes from the three groups were contrastingly drawn. Between the model and control groups one hundred and eighty-six differentially expressed genes were screened, including 150 up-regulated genes and 36 down-regulated genes. Between the Jinkuishenqiwan treatment and model groups two hundred and ninety-nine differentially expressed genes were screened, among which there were 114 up-regulated genes and 185 down-regulated genes. Between the Youguiwan treatment and model groups one hundred and fifty-five differentially expressed genes were screened, among which there were 64 up-regulated genes and 91 down-regulated genes. The twenty-four genes among up-regulated genes in the two treatment groups versus model group, account for 21.05 percent and 37.5 percent in each treatment group respectively, which are mainly the genes related to neurotransmissions/signal transduction, transcription/translation, cellular cycle, hormone and metabolism. However, down-regulated genes in the treatment group versus model group were 35, accounting for 18.92 percent and 38.46 percent in each treatment group respectively, chiefly including the genes association with inflammation/immunization, neurotransmissions/signal transduction. transcription/translation, metabolism and limiting velocity enzyme of dopamine. There were twenty three genes among the up-regulated genes in the model group versus control group but down-regulated in the treatment group versus model group, mainly involving the related genes of inflammation/immunization and neurotransmissions/signal transduction. There were six genes among the down-regulated genes in the model group versus control group but up-regulated in the treatment group versus model group, mainly including the related genes of cellular cycle and structure, neurotransmissions/signal transduction and transcription.Conclusion: The up-regulated genes in the model group mainly involed the genes related to inflammation/immunization, oxidative stress and so on, which made enhancing response to inflammation/immunization and oxidation, wound and impairment, also to agglutination. The phenomenon by above-mentioned is caused by protective response of the organism. In addition, secretion of decreased dopaminergic transmitter can cause dopaminergic disorder. The down-regulated genes included hormone (especially melanin-concentrating hormone (MCH), gonadal hormone, cell cycle, signal transduction/neurotransmission, metabolism (particularly proteometabism), growth and development, (especially cerebral development, sperm development fertization etc). Jikuishenqiwan and Youguiwan may make it markedly up-regulated to notably down-regulated genes of hormone and melanin-concentrating hormone(MCH) for model of mice of kidney-yang deficiency, and promote cellular proliferation, which can inquire into effect mechanism of the two drugs and their differences in genetic level at the same time.