| Section 1 Theoretical study of insomniaInsomnia has not merely reflected the sleep quality that it is difficult,apt to wake up early that the patient falls asleep at night,reduce sleep time obviously,reflected the patient works in daytime at the same time,studies,the low function state of memory and other functions.The current society works,lives,studies the quickening of the rhythm with the aggravation of the competition,the pressure that people face is larger and larger,make insomnia phenomena generally take place,become common disease,the morbidity of insomnia rises notably.Especially among athletes,because the training amount of daily sports is relatively big, and psychological pressure is relatively great,insomnia phenomenon take place often, effect athlete match and training achievement and cause athlete to athletic injury.The medicinal treatment is one of the important methods to treat insomnia.The Western medicine treating insomnia at present mainly remains at phenobarbital.Insomnia will make people feel the head is dizzy,absent minded,lull calmly though the medicine can make people enter the sleep state,but will still present giddy,weak,sleepy,depression after waking up.Most phenobarbital will also aggravate this kind of state,and produced the drug tolerance,addiction,abstinence syndrome etc,the danger that the elderly who often take phenobarbital die is higher than that of normal people.Though Western medicine curing insomnia has gone through long-term development, will be also having new medicines to enter clinically constantly in the future,because the complexity of the mechanism that insomnia has illness coming on,and the characteristic on function of Western medicine,can be in order to think so,the side effect which treats Western medicine of insomnia is that it is impossible to get thoroughly,effective improvement in a short time.In addition,special crowds such as athletes,ere,athlete often face match training and drug test,can't take and Phenobarbital.So,it is an important task of the field of medical and health to offer the medicine with small side effect,with prominent curative effect,with high security.Use the Chinese medicine characteristic and carry on the treatment of insomnia,seek high-efficient square medicine safely,have important research value and realistic meaning.Traditional Chinese medicine by way of its holism concept,prevent before disease, natural,low toxicity,cheap traditional Chinese medicine that the ones that treat in Treatment with syndrome differentiation,can bringing a lot of new thinkings,new methods of insomnia,there are irreplaceable obvious advantages in preventing and curing insomnia.Chinese medicine research of insomnia make a start until " Inner Classic of Yellow Emperor",think insomnia caused by disharmony between Yang and Yin.Treating and beginning with "Treatise on Cold-induced Febrile Diseases " Treatment with syndrome differentiation insomnia of traditional Chinese medicine.Zhang ZhongJing distinguishes and cured insomnia by Xu and Shi.Suanzaoren Decoction is mostly used to treat insomnia after Han Dynasty.Traditional Chinese medicine workers have carried on a large number of clinic and experiment to study to Suanzaoren Decoction in recent years,have made certain achievement,but still have a lot of questions that can't be ignored:(1) Most of clinical practice still confine to facing case summarizing,case report of experience,there are relatively less clinical observation reports of the big sample. Especially in the report of spiritual disease of nervous system,the name of disease of disease can't be integrated with modern medical science,clinical diagnosis with curative effect appraise,lack norm of unifying(2) Medicinal Chemistry study on Suanzaoren Decoction is lack.(3) The Pharmacology study of Suanzaoren Decoction is limited in component,and Toxicology.(4) Be lack of clinical research and experiment,Evidence-Based Medicine and advanced statistics method hardly to carry on extensive and deeper research.Suanzaoren Decoction as clinical classics,clinicl and experiment its study and also at the initial starting stage.Use modern study methods to improve clinical application,it is a key problem to be solved while studying at present.In addition,daily I am mainly engaged in the application of traditional Chinese medicine in sports disease,insomnia phenomenon take place in athletes often,influence athlete match and training achievement and cause athletic injury.Athletes can not use Phenobarbital because of match training and drug test.In order to lighten the impact on athletes' physical and mental health of the insomnia phenomenon,improve athletes' training and result,make athletes more easy and prepare for 2008 Olympic Games(note:This research begins in 2006,and will serve Beijing 2008 Olympic Games) With 2010 Asian Games.Section 2 Study on the mechanism of Suanzaoren Decoctioninterfere insomnia ratsobject:Establish insomnia rats model and study Mechanism of Suanzaoren Decoction.Methods:Adopt 60 SPF rank SD rats,female,weight 200±50g,ranked rats by it is the weigh. Produced the random digit by SPSS,divide 6 group,normal group,model group,Western medicine control group,traditional Chinese medicine control group,Suanzaoren Decoction low dosage group,Suanzaoren Decoction high dosage group.10 every groups.Put all big mice in SPF laboratory,ingest,drink the water freely.Room temperature is 22℃.Keep the natural illumination,adaptability is raised for 2 weeks.At the beginning of testing,give model group physiological saline by the stomach, give the control group of Western medicine phenobarbital by the stomach;The control group of traditional Chinese medicine spurts the kidney and allays excitement pill irritates the stomach;The low dosage of Suanzaoren Decoction,high Suanzaoren Decoction by the stomach for a week.Training rats sets of strength exhaust,train with the Model Y maze experiment After operation described above lasts one week,since every day the second week 8 AM, 350mg/kg abdominal cavity has injected PCPA.Inject PCPA 72h later,observe the body surface characteristic of all rats,kill all rats keeps the brain at -70.Test the brain NO,SOD,5-HT,Glu,GABA,IL-1β,NGF,TNFα,EPGE2.Use SPSS 17.0 to carry on analysis of variance and relevance analysis to the data materials.Result:1.Compared with normal group all insomnia groups,the body surface characteristic has prominent differences.2.Compared with normal group,all insomnia old mouse brain NO level has not obviously changed;Compared with control group,the stable group of Western medicine and group NO of high dosage of sour jujube kernel soup have the tendency to obviously rise;Compared with control group,SOD level obviously reduce(P＜0.05) in big other mouse's brain.3.Compared with control group,big other mouse's cerebral cortex and hypothalamus position amino acid neurotransmission 5-HT content are obviously a bit high(P＜0.05);Compared with normal group,control group 5-HT obviously raises(P＜0.05) traditional Chinese medicine.Compared with control group,big other mouse's cerebral cortexes and amino acid neurotransmission GABA content of the hypothalamus positions are obviously on the low side(P＜0.05);Compared with normal group,large mouse GABA content obviously raises (P＜0.050) all insomnia;Compared with control group of Western medicine,the content of group GABA of low dosage of jujube kernel of acid is on the low side(P＜0.050)4.Compared with control group,big other mouse's cerebral cortex and hypothalamus position IL-1βcontent are obviously a bit high(P＜0.05);There is not obvious difference among Western medicine group,traditional Chinese medicine control group and traditional Chinese medicine group,the group of high,low dosage of soup of sour jujube kernel does not have obvious difference;Compared with control group,cerebral cortex rats and sea hip NGF content are obviously a bit high(P＜0.05);Compared with normal group,NGF content obviously reduces(P＜0.050) all insomnia;There is not obvious difference among Western medicine group,traditional Chinese medicine control group and traditional Chinese medicine group, the group of high,low dosage of soup of sour jujube kernel does not have obvious difference;5.Compared with control group,rats cerebral cortex and TNF-αcontent of hypothalamns position are obviously on the low side(P＜0.05);There is no obvious difference among normal group,Western medicine group,traditional Chinese medicine control group and group of low dosage of sour jujube kernel soup;Sour jujube kernel soup high dosage group TNF - with treat group compare,obviously reduce while being other. Compared with control group,PG-E2 content of stable group and normal group is relatively on the low side(P＜0.05),Other traditional Chinese medicine treat the group and contrast, have obvious difference;Compared with normal group,large mouse PG-E2 content obviously raises(P＜0.050) all insomnia;Traditional Chinese medicine control group and sour jujube kernel are high,there is no obvious difference between the low dosage groups.6.Regression analysis R =0.336,P＜0.05,the equation that the whole variable is: 5-HT=25.29-16.738TNF-0.724NO+0.112NGF;Multivariate analysis of variance there are prominent differences between the vector quantities in every group,F=4.669,P＜0.05.Conclusion1.Adopt rats strength exhaust excise +Y maze test +PCPA abdominal cavity inject PCPA is a method to induce insomnia on rats,2.Insomnia can lead to the fact oxygen free radical level rises in the brain,the Suanzaoren Decoction can remove oxygen free radical,free radical has good protective action of brain on the damage of the nerve cell while lightening the sleep and depriving.3.Insomnia can cause the rats brain stem 5-HT hip to change:Model rats 5 -HT content drop obviously,GABA content rise notably.The soup of sour jujube kernel can reduce GABA content,changes the single ammonia proportion of passing the quality through promoting 5 - HT content in the hypothalamus,to improve the sleep,protect the brain.4.Insomnia can cause sea horse's cell factor to change:Suanzaoren Decoction improve the sleep and protect the brain cell through the content of IL-1βand NGF improving in brain stem and hip;The content of TNF -αprotects the brain cell,improve the content of regulating PG-E2 to improve the sleep to reduce in the brain.5.In general terms,Suanzaoren Decoction treat insomnia is not superior to others groups.6.Regression analysis shows relevance is not close;Multivariate analysis of variance there are not prominent differences between the vector quantities in every treat groups. |
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