Intervention Effect Of Weiyanyihao On Chronic Atrophic Gastritis Precancerous Lesions

Gastric carcinoma is one of the most common tumors.It early detection, diagnosis,and treatment will greatly decrease mortality rate.There is a long time period of precancerous change before the normal mucosa change into carcinoma.Detecting and treating precancerous lesions is a good way to prevent gastric carcinoma.WHO experts indicated that Chronic atrophic gastritis with dysplasia is a precancerous lesion.Traditional chinese medicine has good curative effects on it.My thesis can be divided into two parts.In the part 1,I summarized the cognition of cause and mechanisms of chronic atrophic gastritis in the view of traditional chinese medicine,and review progress of its etiological factors,pathogenesis,treatment of western and traditional medicine.In the part 2,based on replicating animal model of chronic atrophic gastritis,using biochemical,radioimmunoassay,western blott and PCR technique to study effects of Weiyanyihao formula on freeradical,inflammatory factors, activities and expression of MMPZ and TIMP2.1.PurposeTo study effects of Weiyan yihao formula on histopathological changes, freeradical damage,inflammatory factors,activities and expression of MMP2 and its inhibitor TIMP-2 of gastric tissues in chronic atrophic rats with precancerous lesions.Observating serum free radical damage,the expression of inflammatory cytokines,gastric tissue MMP-2 and its inhibitor TIMP-2 activity and gene expression in rats with chronic atrophic gastritis precancerous lesions and intervention mechanism of Weiyanyihao,with a view to reveal pathogenesis of precancerous lesions in chronic atrophic gastritis,as so as treatment effects of Weiyanyihao on chronic atrophic gastritis precancerous lesions to find a new target.2.Method60 SD rats were radomisely devided into 6 groups:(10 animals in each): model group,control group,Weiyanyihao with high-dose,medium-dose and low-dose group and Weimeisu group.Except the control group received intragastrical administration of normal saline,the rest five groups underwent intragastric MNNG and ranitidine hydrochloride capsules administration to be made into CAG models.Afterwards,corresponding medication was administered to each group for 90 days.After the end of treatment,isolating from the serum, using biochemical method to measure SOD and MDA content,using of radioimmunoassay to measure Serum IL-1β,TNF-αcontent;morphological observation at the same time.Using Western blot to measure activity of MMP2 and TIMP2 in gastric tissue,using PCR technic(SYBR Green) to detecte levels of mRNA MMP-2,TIMP-2 expression in rat gastric tissue.3.Results3.1.The model group has significant stomach membrane inflammation,gland atrophy and metaplasia.There is no significant dysplasia.There are significant differences between Weiyanyihao groups and model group.It indicated that Weiyanyihao formula has improvement effect on the pathological changes in CAG。3.2.Compared to the model group,that of SOD level in the three Wei yanyihao groups significantly higher while the contents of MDA is significantly lower,and the difference was statistically significant.But, there was no statistical difference between Weiyanyihao groups and Weimeisu group.3.3.Compared with the control group,serum IL-1βconcentration slightly increased but there is no statistical difference.Compared with the model group,in the treatment group the serum IL-1βcontent is slight decrease but no statistical difference.Compared with control group,serum TNF-αcontent in treatment groups were increased,but only the model group had significantly different.Compared with model group,serum TNF-αin Weiyanyihao with middle and low-dose groups and Weimeisu group were significantly lower,and in Weiyanyihao with high dose group TNF-αwas decreased not obviously. 3.4.Compared with control group,MMP-2 activity in all rats groups were increased in varying degrees but only in model group the increase was most obvious.Compared with model group,treatment with Weiyanyihao and Weimeisu groups MMP-2 activity was significantly lower,and Weiyan yihao with middle dose had the best effect.Compared with the control group,TIMP-2 activity in all groups were decreased in varying degrees but only the model group were significantly different.Compared with the model group,treatment with Weiyanyihao and Weimeisu,TIMP-2 activity was significantly increased,Weiyanyihao with middle dose had the best effect.3.5.In chronic atrophic model rats,MMP-2 RNA expression increased.After treatment with Weiyanyihao and Weimeisu,the expression of MMP-2RNA significantly decreased,Weiyanyihao with middle dose had the best effect.In chronic atrophic model rats,TIMP-2 RNA expression of gastrictissue was reduced.After treatment with Weiyanyihao and Weimeisu,the expression of TIMP-2RNA trend to be increased,but there was no statistical difference.4.Conclusion4.1.Weiyanyihao can improve pathological changes of of experimental chronic atrophic gastritis in rats.4.2.It is suggested that Weiyanyihao can increase the level of serum SOD,decrease the content of serum MDA,which maybe contribute to treatment effect on chronic atrophic gastritis rats.4.3.Weiyanyihao can reduce the inflammatory cytokines TNF-αcontent.4.4.In gastric tissue of rat chronic atrophic gastritis activity of MMP2 is increased and activity of TIMP-2 is reduced,so injury factors are increased and protective factors are reduced,so it increase precancerous lesions.Weiyanyihao and Weimeisu can reduce MMP-2 activity,while increase TIMP-2 activity,so they can reduce the the possibility of gastric precancerous lesions.