Experimental Study On Bushenning Reinforcing Kidney And Promoting Ovulation On Kidney Yang Deficiency Rat

Objective:To observe the effect and mechanism which BuShenNing(BSN) decoction promoted the ovulation of kidney-yang defieiency femalerat model,which replicate by applying hydoreortisone.Methods:The sixty mature female Sprague-Dawley rats were dividedinto six groups radomly:normal contrast group,model contrast group,clomifene group,BSN low,middle and high dosage group.normal contrastgroup used physiological saline,the other groups replicated kidney-yangdefieiency model by muscle injecting hydoreortisone 36mg/kg,for 11day.After 11 day,used the corresponding medicine treatment by 15day.Observe each group the change of rat body weight;the level of serumestrogen(E₂) and progesterone(p);each group the hypothalamus,pituitary,adrenal,ovarian,uterine changed in histomorphological differences by lightmicroscope;ultrastrucure changed of pituitary and adrenal;the expression ofhypothalam,usterine,ovarian estrogen receptor(ER);the expression ofhypothalamus estrogen receptor mRNA(ERmRNA) and ovarian matrixmetalloproteinase-9 Mrna(MMP-9mRNA)Results:â‘ After taken medicine,the weight of rat in BSN middle andhigh dosage group heighten evidently,have significant differerce comparedwith model contrast group and clomifene group(P＜0.01),and superiord toBSNlow dosage group(P＜0.05).â‘¡BSN low,middle and high dosage groupand clomifene group all increased level of the rat serum estrogen andprogesterone,significantly superiored to model contrast group(P＜0.05),were similar to normal contrast group(P＞0.05),clomifene group and BSNhigh dosage group the most significant.â‘¢BSN low,middle and high dosagegroup improvd the structure damage of pituitary and adrenal of deficientkidney yang rat,the higher the dose,the effect of the more obvious;modelcontrast group decreased in number of ovarian growth follicle and luteum,BSN low,middle,high dosage group and clomifene group could increase in quantity of ovarian growth follicle and luteum,BSN middle,high dosagegroup and clomifene group were similar to normal contrast group;BSNmiddle and high dosage group improved the morphology of rat uterinestromal and glandular,were superiored to clomifene group,were similar tonormal contrast group;hypothalamus of each group were observed nosignificant difference by light microscope.â‘£BSN low,middle and highdosage group improvd ultrastrucure damage of pituitary and adrenal,BSNmiddle and high dosage group were superiored to clomifene group.⑤BSNlow,middle,high dosage group and clomifene group all could enhance theexpress of hypothalamus,uterine,ovarian ER,BSN middle and high dosagegroup were similar to normal contrast group(P＞0.05),were superiored toclomifene group(P＜0.05) and model contrast group(P＜0.01).â‘¥BSN low,middle,high dosage group and clomifene group all could enhance the expressof hypothalamus ERmRNA and ovarian MMP-9mRNA,were superiored tomodel contrast group(P＜0.05),BSN middle and high dosage group weresignificant superiored to model contrast group(P＜0.01),were similar tonormal contrastgroup(P＞0.05),BSN high dosage group was superiored toclomifene group in the express of ovarian MMP-9mRNA(P＜0.05)Conclusions:BSN decoction could overall regulation hypothalamus-pituitary-ovarian axis function of kidney-yang defieiency female rat model.BSN decoction could significant improve the weight of rat,level of the ratserum estrogen and progesterone;improve the histomorphology of ratpituitary,adrenal,ovarian,uterine and ultrastrucure of pituitary and adrenal;enhance the express of hypothalamus,uterine,ovarian ER;enhance theexpress of hypothalamus ERmRNA and ovarian MMP-9mRNA.BSNdecoction had the effect of inducing ovulation and recovering the function ofovary,the allover effect was better than clomifene.