The Curative Effects On Treatment Of Genital Herpes By Longdanxiegan Granul And The Effect On Immunological Function Of Dendritic Cells

Objective To investigate the clinical significance of Longdanxiegan Chinese traditional medicine Granule in preventing and curing the Shire type genital herpes.To explore the immunological function of Longdanxiegan Granule,through studying the Dendritic cells extracted from the patients'blood.Methods 1) The 60 patients were randomly divided into 3 groups completely.20 were treated with Aciclovir.20 were with Longdanxiegan Chinese traditional medicine Granule.20 were with Longdanxiegan Chinese traditional medicine. Observed the integral of clinical symptoms, clinical efficient rate, and the recurring rate respectively before and after the treatment.2) Peripheral blood mononuclear cells (PBMC) were separated from heparinized venous blood by stimulating factor GM-CSF,IL-4,TNF-α. The Dendritic cells were harvested for phenotypic analysis by flow cytometry.Then some phenotype changes such as CD11c,CD80,CD83,CD86,HLA-DR and HLA-ABC of DCs were observed.3) RNA Of the dendritic cells was extracted by Trizol. The levels of mRNA for TLR1-TLR9 were examined by fluorescent quantitative RT-PCR.Result 1) The integral of clinical symptoms were improved obviously after theatment for three groups.There was no significant difference in clinical rate for the two Chinese traditional medicine groups. The group treated with Longdanxiegan Chinese traditional medicine Granule showed a lower recurring rate than the Aciclovir group.2) The expression of CD11c,CD80,CD83,CD86,HLA-DR and HLA-ABC on DCs were higher in patients than that in healthy controls(P＜0.05). After theatment, the expression of these molecules were regulated, there were no significant differences between patients and controls(P＞0.05).3) The expressions of TLR3,TLR4,TLR7,TLR8 and TLR9 mRNA were significantly higher in patients than that in healthy controls(P<0.05);but the expressions of TLR1,TLR5 and TLR6 mRNA were markedly lower than that in controls(P＜0.05).After theatment, there were no significant differences in the expressions of the TLR1-TLR9mRNA between patients and controls(P＞0.05).Conclusion Longdanxiegan Chinese traditional medicine Granule is effective for the Shire type genital herpes. Longdanxiegan granule could adjust the expressions of TLRs on peripheral blood leucocyte in patients with genital herpes and play a key role in initiating innate immune responses to viral pathogens.