The Protection Of The YeMingKeLi On Photoreceptor Degeneration Of Retinitis Pigmentosa

Retinitis pigmentosa(RP) is a hereditary retinal degenerative disease. This paper researches treating the RP with "Yeming grain" from three aspects which are the theory studying,the clinical research and the empirical study, as well as explores the possible mechanism of the qi and kidney tonics and blood-activating and stasis-removing drugs "Yeming grain" treating the RP.The morbility of the RP possesses genetic predisposition. The cause is inherent deficiency,deficiency of the liver and kidney,which results in asdthenic splenonephro-yang,decline of vital gate-fire,qi and blood disorder,eye collaterals stagnation. This is a slow-moving patho- process which is based on void, then results in stagnation,and develops to void nipping stagnation at last. My tutor Shuhua Ding invents the prescription of "Yeming grain" to treat the RP according to her clinical experience. This prescription aims directly at the etiopathogenesis of the RP,and establishes the principle of removing stagnation and filling void.The clinical observation of the patient who is treated by "Yeming grain" and the analyses of eyesight, visual acuity,and bright-flash electroretinogram indicate that "Yeming grain" is effective."Yeming grain" can not only relieve the progression of disease but improve each index. The effective rate is 84.5%.In the first text,we give the treatment to rats,then observe and analyze the effect of different dosages on the microcirculation,cleaning up the free radicle in vivo,accommodating the immunity and the rat's hemorheology.Results:"Yeming grain" can improve the microcirculation,step down the blood viscosity, promote blood flow and remove stagnation;it can improve the SOD activity of rat's blood,step down the value of MDA,and enhance the cat's capability of cleaning up the free radicle in vivo and the accumulation of lipid peroxidate;it also have accommodative effect on the immune function.To go deep into exploring the mechanism of "Yeming grain" treating the RP and to determine whether tetram ethylpyrazine hydrochlorde has an efficacy on hereditary retinitis pigmentosa of rds mice by the observation of the photo receptor cells. Nine rds mice of 4 week old were randomly divided into three groups, "Yeming grain" group(4 mice),"qujudihuangwan" group(3 mice) and control group(2 mice).All mice were sacrificed after 28d with eyes being immediately enucleated and fixed in 10% form aldehyde in PBS. Routine pathologic process was done and sections were stained by hematoxylin and eosin(HE).The layer numbers of retinal photo receptor cells at equator posterior center was counted under light micro scopy. Nine rds mice of 12week old did as so on.Result from light microscopy showed that the layer numbers and numbers of retinal photo receptor cells in the tetram ethylpyrazine treatment group were increased in rds mice,compared with the control group(P<0.05).Conclusion from light microscopy and electron microscope suggest that tetram ethylpyrazine has a significant efficacy to the retinal photo receptor cells of rds mice.