| In this research,The Evodia rutae-carpa(Juss) Benth-Angelica sinensis(Oliv) Diels herbal pair of the Wen-Jing Decoction will be studied on the basis of their effectiveness of demulcent and their effective ingredients.On the basic of determination the effective fraction of the decoction of Evodia rutae-carpa(Juss) Benth-Angelica sinensis(Oliv) Diels herbal pair,the serum pharmacology and pharmacochemistry methods were used,and the demulcent effect and the fingerprint chromatogram was be researched.The relation of the effect and the chromatogram was analyzed to explore the demulcent material foundation of the herbal pair.Purpose:Tracing of the theory of compatibility and profile-effect of Evodia rutae-carpa (Juss) Benth- Angelica sinensis(Oliv) Diels herbal pair by the pharmacy and the pharmacodynamics.Material and method:Material:Evodia rutaecarpa(Juss) Benth and Angelica Sinensis(Oliv.) Diels were purchased from Hebei Anguo;AR2140 electronic analytical balance;AS3120A ultrasonic extractor; U3010-type UV-Vis Spectrophotometer;Agilent1100 United States-based liquid chromatography system,METTLER AB135-S balance 1/100000;liquid chromatography with acetonitrile to pure water for the re-distilled water;all other reagents is analysis pure.Method:In the pharmaceutical aspects,to determine the content of alkaloids,evodiamine and rutacarpine,ferulic acid of the three different preparation sample by UV-Vis spectrophotometry and HPLC;and to determine the content of the same target of the different matching proportion.In the Pharmacodynamic aspects,to study the analgesic effects of the same sample by writhing test and the hot plate test in mice.So,we screen out the best distill method is alcohol merge and the best matching proportion is 6:4.Orthogonal design was applied to optimize the extract condition.Orthogonal table L9(34) in the experiment of extracting was chosen.The influence factors of extraction times,extraction hours,ethanol concentration,solvent multiple were selected by orthogonal test with Alkaloids,Evodiamine,and Ferulic acid as the compositive index.The compositive index above was determined in different preparation samples.The quality standards and fingerprint chromatogram were established by TLC and HPLC.The HPLC fingerprints were obtained from Agilent Eclipse instrument.The HPLC separation was performed on a C18 analytical column gradient eluted with acetonitrile-0.1%H3PO4.The temperature of column was 25℃.The UV detection wavelength was 287 nm.By Isolated rat uterus experiment,we observe the activities effects of the extraction on the isolated rat uterus;By rat reproductive endocrine system regulation experiments,we study the influence about the E2 and PGF2α on rat of the extraction with Elisa. Studying on the correlation of the Analgesic effect and the fingerprint chromatogram.To establish the fingerprint of Evodia rutae-carpa(Juss) Benth- Angelica sinensis(Oliv) Diels herbal pair by HPLC.The chromatographic condition was same to the former.Correlating analysis was made to discern the interrelationship between each peak and the Pharmacodynamic data of the analgesic effect o in different proportion.Results:1.By UV-Vis spectrophotometry and HPLC,and the analgesic effects,we get the best distill method and the best matching proportion.It is ethanol combined extract and 6:4 matching proportion.2.By Orthogonal design with Alkaloids,Evodiamine,and Ferulic acid as the compositive index,was get the optimize the extract condition.It is 70%ethanol(8 times),extraction times(2 times),extraction hours(2h)3.The quality standards was established by TLC and HPLC.The fingerprint chromatogram were established by HPLC.Three batch samples shows that The minimum content of asafetida is 0.983mg·g-1,evodiamine and rutacarpine separately is 2.350mg·g-1,2.101mg·g-1.Tentative,the content of asafetida of this product is higher than 0.90mg·g-1; evodiamine and rutacarpine separately is higher than 2.0mg·g-1,2.0mg·g-1.4.The extract had effects on the contraction of normal isolated uteruses.The uteruses could be distinctly inhibited by medium and high concentration of the extract.The excitement induced by oxytocin could be concentration-dependently inhibited by the extract.It could remarkably inhibit contraction frequency,amplitude and activity of uterine smooth muscle induced by oxytocin.5.In bivariate correlating analysis,the Inhibition rate was found having good interrelationship with the peak3,4,7,11,14,15,16(p＜0.05),In enter regression analysis,the peak 3,4,6,12,13,14,16,17 were entered(p＜0.05).In stepwise regression analysis,the peak 6,7,10,13,14 were entered(p＜0.05).Conclusion:1.By Orthogonal design,we get the optimum extraction technology of Evodia rutae-carpa (Juss) Benth- Angelica sinensis(Oliv) Diels herbal pair.The optimum extraction process is reliable and the extraction ratio of active components is high.2.By TLC and HPLC,we establish the quality standards of Evodia rutae-carpa(Juss) Benth-Angelica sinensis(Oliv) Diels herbal pair.The method is simple and accurate,it can be used in the quality control.3.The fingerprint chromatogram of Evodia rutae-carpa(Juss) Benth- Angelica sinensis(Oliv) Diels herbal pair were set up.4.The extact of Evodia rutae-carpa(Juss) Benth- Angelica sinensis(Oliv) Diels herbal pair can relax uterine smooth muscle of virginal rats.5.By bivariate correlating analysis and regression analysis,we inferred that the 3,4,7,10,11,13,14,15 and 16 peak have good interrelationship with the inhibition rate of rat uterine smooth muscle.
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