The Study Of Radix Aconite Lateralis Prepapata Compatibility Radix Glycyrrhizae, Radix Et Rhizoma Rhei And Rhizoma Zingiberis Control Material Foundation Of Herb Property Regulation

Objective:In the direction of Chinese Medicine theory, according to Radix Aconite Lateralis Prepapata mainly in the use of decoction solution, choose the traditional use of the Crude Radix aconiti lateralis preparata and now mainstream films White prepared lateral root of aconite, Black prepared lateral root of aconite as the research object, research Radix Aconite Lateralis Prepapata with Radix Glycyrrhizae, Radix et Rhizoma Rhei and Rhizoma Zingiberis compatibility different ratio (1:0.5,1:1,1:2) after the decoction of its ingredients target changes regular pattern, Combined with mass spectrometry results, From the chemical composition perspective reveals the Radix Aconite Lateralis Prepapata through the combined enhanced effect, reducing toxic mechanism. At the same time provide a scientific basis of Chinese herb control property regulation.Methods:By ultraviolet spectrophotometry (UV), high-performance liquid chromatography (HPLC), determination of target ingredients of Radix Aconite Lateralis Prepapata(total alkaloids, monoeste-diter-penoidalkaloids, diester-diterpenoid alkaloids); Radix Glycyrrhizae(flavonoids,Saponins,Polysaccharide); Radix et Rhizoma Rhei(anthraquinone,anthraquinone-bound, Free anthraquinones, Polysaccharide), Rhizoma Zingiberis(capsaicin). Using electrospray ionization mass spectrometry (ESI) study the aconitine, mesaconitine, hypaconitine, and its hydrolysis solution, and Crude Radix aconiti lateralis preparata compatibility Radix Glycyrrhizae, Radix et Rhizoma Rhei and Rhizoma Zingiberis decoction solution. According to m/z confirm the molecular ion peak.Results:1. Radix Aconite Lateralis Prepapata compatibility Radix Glycyrrhizae After water decoction, the content of total alkaloids increased slightly, about 2%; monoeste-diter-penoidalkaloids and diester-diterpenoid alkaloids content decreased obviously, the maximum drop of 63% and 83%, and directly proportional to compatibility the amount of Radix Glycyrrhizae. Flavonoids and saponins were decreased obviously, the maximum drop of 19% and 55%, and directly proportional to compatibility the amount of Radix Aconite Lateralis Prepapata.2. Radix Aconite Lateralis Prepapata compatibility Radix et Rhizoma Rhei After water decoction, the content of total alkaloids, monoeste-diter-penoidalkaloids and diester-diterpenoid alkaloids decreased obviously, the maximum drop of 47%,64% and 92%, and directly proportional to compatibility the amount of Radix et Rhizoma Rhei. Anthraquinone, anthraquinone-bound and free anthraquinones were decreased obviously, the maximum decrease of 64%,57% and 75%, and directly proportional to compatibility the amount of Radix Aconite Lateralis Prepapata.3. Radix Aconite Lateralis Prepapata compatibility Rhizoma Zingiberis After water decoction, the content of total alkaloids, monoeste-diter-penoidalkaloids increased, the maximum increase of 31% and 9%, and directly proportional to compatibility the amount of Rhizoma Zingiberis; diester-diterpenoid alkaloids content the same as the original. At the same time, capsaicin content increased,the maximum increase of 15%, and directly proportional to compatibility the amount of Radix Aconite Lateralis Prepapata.4. Radix Aconite Lateralis Prepapata compatibility herb MS study From the aconitine, mesaconitine, hypaconitine hydrolysis of 10 minutes on the mass spectrum figure, we can see the product of they C_8bit loss a acetyl,it's Benzoylaconine; From they hydrolysis of 20 minutes on the mass spectrum figure, we can see the product of they C_14bit loss a benzoyl, it's aconine; From the Radix Aconite Lateralis Prepapata decoction solution have aconine, monoeste-diter-penoidalkaloids,diester-diterpenoid, lipid alkaloids, total 15,from the compatibility Radix Glycyrrhizae and Radix et Rhizoma Rhei, we observed that the three kinds of new lipid alkaloids.Conclusion:The experiment results was based on material foundation evidence of Aconite can change herb property regulation through the compatibility herb, and mutual influence, compatibility Radix Glycyrrhiza use ping xing, ease Aconite big hot and toxicity; compatibility Radix et Rhizoma Rhei use han xing, except reducing Aconite toxicity; also ease Radix et Rhizoma Rhei strong role of diarrhea, variable hanxia to wenxia; compatibility Rhizoma Zingiberis use wen xing, together enhance the huiyangjiuli effection. In a word, compatibility change herb property regulation was eld come from the clinical practice, are unique theory of Chinese herb, of clinical doctors today have a very important guiding significance...