Study Of The CD133 Positive Cells In FADU Cell Line

Hypopharyngeal cancer is one of head and neck malignant tumor, which is also one of the highest level of malignant tumor in upper digestive tract and upper respiratory tract. The mechanism isn't clear.The reasons of poor prognosis include: local recurrence, lymphatic metastasis and metastasis.Because of poor prognosis,and may affect the quality of patient's life severely, hypopharyngeal cancer is one of the research priorities in otorhinolaryngology.Cancer stem cells is a new theoretical perspective, the new view is that only a very small amount of tumor cells have stem cell-like role,.they have potential for unlimited self-renewal and promote tumor formation, The cells play a decisive role in tumor growth. Such cells have a strong resistance to radiation therapy and chemotherapy, and may be related to immune escape, the growth of abnormal signal transmission and many other important feature of the tumor. At present, cancer stem cells have been identified in brain neoplasms, leukemia, breast cancers laryngeal cancer and lung cancer through an experimental strategy. From the current research results, the different tumors, as some biological characteristics have in common, such as the unlimited self-renewal potential and differentiation capacity, but the cancer stem cell markers on its surface is not the same, which prompt us to the need for accordingly different tumor research, find the different tumor markers of cancer stem cells in different tumors.In recent years, many studies have confirmed that, CD 133 is one of the important markers for cancer stem cells,. CD133 is an early antigen whose molecular weight is 120 kDa, with five transmembrane glycoprotein. In 2000, the antigen was officially named CD133 in the United Kingdom at the 7th session of the International seminar on human leukocyte differentiation.CD133 is a normal surface markers for hematopoietic stem cell.one of its important features is:CD133 expresses with cell differentiation and down rapidly.It selectively expressed in stem cells in the bone marrow and peripheral blood and endothelial progenitor cells, but never in mature endothelial cells.CD133 is an important separation and identification marker for hematopoietic stem or progenitor cell.Invasion capacity is a sign of behavior characteristics of malignant tumors.It is one of the important direction in current study of the characteristics of malignant tumors. Transwell chamber is currently using in vitro for the study of tumor cell invasion and metastasis behavior.This study is intended to CD133 as a sorting marker of cell lines from human FADU cell line.CD133-positive cells were selected to study the distribution of their number, morphological characteristics, and intends to study the FADU cell lines CD133-positive cells in the invasion capacity through Transwell chamber. PartⅠStudy of the expressions of CD133 in FADU cell lineObjective:Study of the expressions of CD 133 in FADU cell line.Methods:FADU cells are cultured and passaged, the expression of CD133 was studied by immunocytochemistry and flow cytometry methods.Results:CD133-positive cells accounted for only a small number of the FADU cells by mmunocytochemistry method, while the ratio of CD133-positive cell in FADU cell line is only 2.01% in the use of flow cytometry method.Conclusion:The number of CD133-positive cells accounted for a small number of cell lines FADU. PartⅡCells sorting of CD133-positive in Hypopharyngeal FADU cell lineObjective:Applied with the magnetic cell sorting(MACS) technology, we purified CD133-positive cells from a FADU cell line and identified.Methods:FADU cells are cultured and passaged, we purified CD133-positive cells from a FADU cell line by magnetic cell sorting(MACS) technology and identified.Results:Application of magnetic cell sorting(MACS) technology, may be a good choice for purifying of CD133-positive cells, purity of the CD133-positive cells after sorting is 91.2%.Conclusion:Application of magnetic cell sorting(MACS) technology, may be a good choice for purifying of CD133-positive cells PartⅢInvasion of the CD133 positive cells in FADU cell lineObjective:To investigate the Invasion of the CD 133 positive cells in FADU cell line.Methods:In our study, Immunomagnetic beads were applied to purify CD133-positive cells in FADU cell line. Invasion of CD133-positive, CD133-negative cells were was measured by Transwell experiment.Results:The cell number that infiltrated Transwell membrane in CD133-positive group (54±2.3) was significantly higher than that in CD133-negative group (17±1.1).Conclusion:CD133-positive cells in FADU cell line show higher Invasion.