The Study Of The Experession Of MCT1 And CD147 In Human Hepatocellar Carcinoma And The Influence Of The Anti-sense Reconstructed Vector Of CD147 And MCT1 To HEPG2

Objective:The study was to explore the expression level of MCT1 and CD 147 in human hepatocellular carcinoma,espacially the changes of expression level in different clinical phases and pathological grades by using immunohistochemical localization.MCTl and CD 147 gene anti-sense reconstructed vector were used to influence the experession of MCT1 on HEPG2,so that the lactic acid metabolism will be disturbed and cell growth will be delayed.Methods:60 sections were obtained from patients with hepatocellular carcinoma. 50 cases with normal liver tissues were included into the control group.All sections were immunohistochemcally stained to observe the difference of CD147,MCT1 expression level between different pathological grade.Two kinds of anti-sense reconstructed vector (CD147 and MCT1)were transfected into the HEPG2.The cells were divided into three groups:HEPG2,HEPG2-PCIneo and HEPG2-PCI-MCT1(CD147).After transfection, the western-blot was used to detected the change of MCT1 expression. The pHi, intracellular lactic acid content and the tumor proliferation were recorded for comparison.Results:The expression rates of CD147 and MCT1 in hepatocellular carcinoma tissues were 76.67% and 81.67%, respectively, which were significantly higher than those in normal liver tissue (P< 0.01). The expressions of CD147 and MCT1 were closely correlated with degree of differentiation and metastasis, but no association was observed with Child grades. There was a significant correlation between the expressions of CD147 and MCT1 in hepatocellular carcinoma (P< 0.01).In cell culture trial,the expression of MCT1 were significantly changed after transfection of MCT1 gene anti-sense reconstructed vector.And after the transfection of CD147 gene anti-sense reconstructed vector,the MCT1 expression was invalid. After transfection, the pHi of HEPG2 was decreased.The lactic acid content increased,grow belocity retarted,doubling time increased(P<0.05).Conclusion:The overexpressions of CD147 and MCT1 may be used as one of the objective marks to estimate the behaviors of hepatocellular carcinoma.The CD147,MCT1 anti-sense reconstructed vector could downregulated the expresion of the MCT1.And the hepatocellular carcinoma cell's lactic acid metabolim could be disturbed and cell growth could be delayed. We have found a new possible path for hepatocellular carcinoma therapy,but the detailed mechanism between MCT1 and CD 147 is remain uncertain and in vivo trial is necessary to research.