| AimsTo study the production and distribution of Pigment Epithelium Derived Factor (PEDF) in human clear cell renal cell carcinoma and normal kidney tissues.MethodsThe expression of PEDF mRNA values in human clear cell renal cell carcinoma and normal kidney tissues were measured by real-time reverse transcription polymerase chain reaction (Real-time PCR). The expression of PEDF protein in human clear cell renal cell carcinoma and normal kidney tissues were detected by Western blot analysis. The expression and distribution of PEDF and microvessel (positive staining for CD34) in human clear cell renal cell carcinoma and normal kidney tissues were detected by immunohistochemical method.ResultsPEDF were expressed in human normal kidney tissues. The expression of PEDF mRNA was lower in human clear cell renal cell carcinoma than normal kidney tissues (P<0.05). The expression of PEDF protein was lower in human clear cell renal cell carcinoma than normal kidney tissues (P<0.05). The microvessel density got more and more in normal kidney tissues, PEDF-positive clear cell renal cell carcinoma and PEDF-negative clear cell renal cell carcinoma (P<0.05).ConclusionsThe lower expression of PEDF in human clear cell renal cell carcinoma may contribute to its pathogenesis and angiogenesis. AimsThe purpose of this study was to investigate the inhibitory ability of PEDF in clear cell renal cell carcinoma in vivo.MethodsNude mice were injected with 5*106 human renal cell carcinoma cell line 786-0 cells into the right flank. we conducted successfully the subcutaneous xenotransplantation of renal cell carcinoma. After 4 weeks, the mice were selected randomly to receive phosphate-buffered saline (PBS) only as control (n=6) or rPEDF protein (n=6) at a dose of 1μg/200μl via intratumoral injections. The tumor was monitored every 2 days using calipers. Four days after treatment, microvessels (positive staining for CD34) in tumors were detected by immunohistochemical method.ResultsAt day 4 after treatment, tumor volume in treated with rPEDF (1202.44±56.34mm3) was significantly (p<0.05) smaller than that in treated with PBS group(1672.23±65.28mm3), with 28% reduction in tumor size compared with control group. Microvessel density in treated with rPEDF(72.83±12.34 per field) was significantly (p<0.01) less than that in treated with PBS group(25.67±8.56 per field), with 65% reduction in MVD compared with control group.ConclusionsRecombinant PEDF could inhibit renal cell carcinoma angiogenesis in vivo. Its ability to halt the growth of renal call carcinoma maybe due to its anti-angiogenic activity. AimsThe purpose of this study was to investigate the prognostic significance of Pigment epithelium-derived factor (PEDF) in human clear cell renal cell carcinoma tissues.Patients and methodswe investigated the expression of PEDF and its correlation with clinicopathological features in 203 patients who underwent radical nephrectomy for CCRCC. Expression of PEDF and CD34-positive vessels (microvessel density:MVD) in tumor samples was examined by immunohistochemistry. The relationship between PEDF expression and clinicopathologic features including tumor diameter, MVD, and survival was evaluated by univariate and multivariate analyses.ResultsOf 203 patients,116 cases (57.1%) were positive for PEDF. A significant association was found between PEDF expression and high microvessel density (p= 0.012). PEDF expression was inversely correlated with grade (p= 0.004) and pT stage (p= 0.001). Multivariate analysis using the Cox regression model indicated that PEDF expression was an independent favorable prognostic factor (risk ratio,4.105; p= 0.001). However, no significant correlation was found between PEDF expression and age, gender, tumor diameter or lymph node invasion.ConclusionsOur findings suggest that PEDF may be a favorable prognostic indicator in patients with CCRCC. |
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