Role Of Allogeneic Mesenchymal Stem Cells In The Mechanism Of Renal Capillary Leakage With Severe Acute Pancreatitis

Objectives: To investigate the role of allogeneic mesenchymal stem cells(MSCs) inthe mechanism of renal capillary leakage with severe acute pancreatitis(SAP).Methods：1.The SD rats were received induction of renal injury with SAP. Then the bloodspecimens were collected for amylase(AMY), creatinine(Cr), urea nitrogen(BUN),interlukin-1β(IL-1β), and tumor necrosis factor α(TNF-α) analysis, the tissuespecimens were harvested for morphological studies, ultrastructural examination;2.The cultured MSCs were identified by cell morphological detection, growth curve,flow cytometry and directional differentiation method;3.The SAP-induced animals were treated with MSCs. Then the blood specimens werecollected for AMY, Cr, BUN, IL-1β, and TNF-α analysis, the tissue specimens wereharvested for morphological studies, ultrastructural examination, and the changes ofaquaporin1(AQP1), matrix metalloproteinase9(MMP-9), vasodilator-stimulatedphosphoprotein(VASP) were examed by using immunohistochemistry examination,qRT-PCR, and Western-blot analysis, respectively.Results:1.The induction of renal injury with SAP could be done by the method of thetaurocholate-induced with ligation of biliopancreatic duct. The levels of ascites output,AMY, and serum AMY, Cr, BUN, TNF-α、IL-1β were rose, the injury of thepancreatic and renal capillary endothelial barrier were aggravated over time;2.The cells were cultured by whole the marrow differential adherence method with thedensity gradient centrifugation method expressed high levels of CD29/90and lowlevels of CD34/45, and could be induced differentiate into the types of osteoblast andlipoblast; The cells labeled by chlormethylbenzamido-1,1-dioctadecyl-3,3,3’,3’-tetramethylin-docarbocyamine(CM-Dil) expressed high fluorescence, andhigh-activity; 3.The MSCs could inhibit the elevation of serum TNF-α、 IL-1β, reverse thedown-regulation of AQP1and VASP and up-regulation of MMP-9in pancreas andkidney, and alleviate the damage of the pancreatic and renal capillary endothelialbarrier.Conclusions: The MSCs could be located in the damaged portion of the pancreas andkidney, inhibited the inflammatory factors activation, regulated the balance ofvascular endothelial cells structure related proteins, relieved the damage of capillaryendothelial barrier, and ultimately reduced renal injury with SAP.