Removing The Heart Yang Treatments For Heart Yang Deficiency Type Molecular Mechanism Of Chronic Heart Failure Rats

Objective:This study was to establish heart-Yang deficiency of chronic heart failure model of male rat by adriamycin(ADR),and molecular mechanism research of Shenfu Tang warming heart-Yang on heart-Yang deficiency of chronic heart failure.Method:The SD male rats were randomly divided into two groups,normal control group20rats, model group,20rats, model group were injected in intraperitoneal with ADR,4mg/kg,1ml/100g, once a week, a total of6weeks, normal group were injected with the same volume of saline.Determine the success of the modeling,the model rats were randomly divided into three groups, model group,digoxin group,shenfu tang group,each group has20rats,add normal control group, a total of four groups.positive group was given digoxin ig gavage,0.1mg/kg,1ml/100g, once a day a total of7weeks; Shen Fu Tang group was given Shen Fu Tang decoction ig,1.875g/kg, lml/100g,once a day, a total of7weeks; Normal group and model group were given the same volume of saline ig once a day, a total of7weeks. Rats were observed daily and mortality in general, the experimental animals weighed weekly, weighed after the last dose, Before death with0.6%sodium pentobarbital anesthesia, lml/100g,60mg/kg intraperitoneal injection, supine on the operating table and fixed detection of left ventricular function, and then take the samples. observed rat heart morphology, body cavity effusion, cardiac index determination, heart histopathology sections, SOD, MDA,GSH-Px、NO、NOs、ET-1detection. Detection of serum AST, LDH, CK, Detection of plasma ANP content, Myocardial tissue in CaN, NFAT3, Ca2+, Na+-K+-ATP enzyme, Ca2+-ATP enzyme activity.Result:After therapy,compared with model group, shenfu tang group can gain weight, decreased cardiac index, increased heart function, cardiac morphology were significantly improved, Shen Fu Tang can improve the myocardial cells in the SOD and GSH-Px activity and decreased MDA content, decreased serum AST, LDH, CK activity,reduce the content of plasma ANP, increased the content of myocardial tissue NO、NOs,reduce the content of myocardial tissue ET-1,increased activity of myocardial tissue Na+-K+-ATP enzyme, Ca2+-ATP enzyme, the content of CaN in shenfu tang group were obviously lower, The calcium content increased significantly.In the Rats myocardial tissue,the light density ratio of dephosphorylation NFAT3/β-actin lower than model group (P<0.05or P<0.01).Conclusion:1.Shenfu tang can scavenge oxygen free radicals,degradation of lipid peroxides,protect myocardial cells from free radical damage.2. Shenfu tang can improve myocardial NO/ET imbalance,restore myocardial vascular endothelial function.3Shenfu tang can improve energy metabolism,lower intracellular Ca2+concentration,inhibit calcium overload.4Shenfu tang can effectively inhibit the activity of CaN-NFAT3signal transduction pathway, Prevent cardiac hypertrophy and heart failure development and maintenance of its normal function,Ultimately be effectively restored heart function,improve the symptoms of heart-yang, heart failure have been restored.Shen Fu Tang prevent and treat heart yang deficiency of chronic heart failure from a number of aspects.