Mechanism Of Histone Deacetylases SIRT1in Different Types Of Atrial Fibrillation

Part ⅠExpression of histone deacetylases SIRT1and relationship between SIRT1and atrial fibrosis in atrial fibrillation of rheumatic heart diseaseObjective To observe the expression of SIRT1, p53and find the relationship between SIRT1, p53and atrial fibrosis in atrial fibrillation of rheumatic heart disease, then find the mechanism of SIRT1in atrial fibrillation.Methods38patients with rheumatic heart disease were taken as the study group,they were divided into two groups:AF group (atrial fibrillation, n=25) with atrial fibrillation longer than six months and SR group (sinus rhythm, n=13). The right atrial appendage tissue was obtained during heart valvular surgery. The expression of SIRT1in atrial tissue was detected by real-time quantitative PCR and immunohistochemical assay,the expression of SIRT1and p53protein were detected by immunohistochemical assay,the atrial fibrosis was identified by masson staining,the cardiac structure was measured by the transthoracic echocardiography.Results Compared with the SR group, the atrial fibrosis was more serious and the cardiac volume factor was higher in the AF group (P<0.05),the expression of SIRT1mRNA in the atrium was significantly increased in AF groups (P<0.01),the expression of SIRT1protein in the atrium was significantly increased in AF groups (P<0.01). Compared with the SR group,the expression of p53protein in the atrium was also significantly increased in AF groups (P<0.05). Linear correlation revealed that SIRT1mRNA and protein had negative correlation with atrial fibrosis, p<0.05. Linear correlation revealed that p53expression had no correlation with SIRT1, p>0.05. Linear correlation revealed that SIRT1expression had no correlation with atrial dimension, p>0.05. Linear correlation also revealed that SIRT1mRNA and protein had negative correlation with the duration of AF(P <0.05). Conclusion SIRT1expression was found increasing in atrial fibrillation SIRT1maybe effect the atrial fibrillation to inhibit the atrial fibrosis.Innovations and limitationsSIRT1mRNA and protein expression were tested in body atrial tissue for the first time in this study;This is also the first study to detect the relationship between SIRT1and atrial fibrillation of rheumatic heart disease.We found that SIRT1expression of patient with atrial fibrillation was more increased than that of patients with sinus rhythm for the first time;We also found that SIRT1expression and atrial fibrosis was negatively correlated, SIRT1could inhibit atrial fibrosis, and the inhibition was negatively correlated with the duration of atrial fibrillation for the first time.This is the first time that we found that SIRT1expression and p53had no significant correlation.This study was only including the patients with rheumatic heart disease, the results were limited. Part ⅡExpression of histone deacetylases SIRT1in the peripheral blood mononuclear cells and relationship between SIRT1,Oxidative Stress and inflammation in atrial fibrillation with hypertiensonObjective To observe the expression of SIRT1in the peripheral blood mononuclear cells and relationship between SIRT1、Oxidative Stress and inflammation in atrial fibrillation with hypertension.Methods58patients with atrial fibrillation and hypertention were taken as the study group,they were divided into two groups:the AF1group(with persistent atiral fibrillation,n=33)、the AF2group(with paroxysmal atiral fibrillation,n=25).Another30patients without atrial fibrillation were the SR group(with sinus rhythm n=30).All the patients should note age, gender, smoking history and so on.The blood sample was took the next morning after hospitaled.The expression of SIRT1protein in the peripheral mononuclear cells was measured using realtime PCR, and Oxidative Stress was estimated by MDA, inflammation was estimated by hs-CRP of the blood sample.Results SIRT1expression was found in the three groups,compared with the SR groups, the expression of SIRT1mRNA in the leukomonocyte of the blood sample was significantly increased in the AF2groups (P<0.01),.however the expression of SIRT1mRNA was not higher in the AF1groups (P>0.05), compared with the AF1groups, the expression of SIRT1was significantly increased in the AF2groups (P<0.01).Compared with the SR groups,the MDA of the blood sample was significantly increased in the AF1groups (P<0.01),.however not higher in the AF2groups (P>0.05), compared with the AF2groups, the MDA was significantly increased in the AF1groups (P<0.01).Compared with the SR groups,the hs-CRP of blood sample was significantly increased in the AF1groups (P<0.01),.however not higher in the AF2groups (P>0.05), compared with the AF2groups, the hs-CRP was significantly increased in the AF1groups (P<0.01).linear correlation revealed that SIRT1expression had significantly negative correlation with MDA,p<0.05,and linear correlation revealed that SIRT1expression had no correlation with hs-CRP(P>0.05). Linear correlation also revealed that SIRT1mRNA and protein had negative correlation with the duration of AF(P<0.01).Conclusion SIRT1expression was found increasing in paroxysmal atrial fibrillation with hypertention, SIRT1could inhibit the Oxidative Stress and effect the atrial fibrillation.Innovations and limitationsSIRT1mRNA expression was tested in human peripheral blood cells for the first time in this study;This is also the first study to detect the relationship between SIRT1and atrial fibrillation of hypertension.We found that SIRT1expression of patient with atrial fibrillation was more increased than that of patients with sinus rhythm for the first time especially the patients with paroxysmal atrial fibrillation;We also found that SIRT1expression and oxidative stress was negatively correlated, SIRT1could inhibit oxidative stress, and the inhibition was negatively correlated with the duration of atrial fibrillation for the first time.This is the first time that we found that SIRT1expression and hsCRP had no significant correlation.This study was not including the deeper mechanism of SIRT1with atrial fibrillation,the results were limited.