Based On The Social Psychological Factors On The Ovarian Reserve Function Decline, Machine, Card, Put To Study

Objective:To study the relationship between social-psychological factors and diminished ovarian reserve, Explore the syndrome distribution rules of diminished ovarian reserve, and evaluate the efficacy of DOR by Tranditional Chinese Medicine.Methods:①Assess the psychosocial situat ion of diminished ovarian reserve patients by clinical research;②Sexually mature female Wistar rats were randomly divided into experimental and control groups. The simulation of chronic mental stress with chronic mild unpredictable stress (CUMS) depress the rats, Detect estradiol (E2) and follicle stimulating hormone (FSH) level in serum, as well as ovarian Biopsy; and caspases-3, Apaf-1expression levels of ovarian were detected by western blot, to explore the pathogenesis of DOR with psychosocial factors;③Analysis the frequency of Chief complaints and symptoms of diminished ovarian reserve patients, to explore the syndrome distribution rules;④Compared the symptoms score before and after treatment,to observe the efficacy DOR by TCM.Results:①There are more smokers, and mental workers,with more consciously work pressure in diminished ovarian reserve patients,compared with other females (P<0.05). The total and mean score, number of positive items of SCL-90were higher than healthy women (P<0.05). DOR patients acts more interpersonal sensitivity, depression, anxiety, paranoia and nervousness (P<0.05).②Serum E2levels decreased, and FSH levels increased in experimental group, compared with the control group, with significant differences (P<0.05). While in the experimental group, all levels of the number of follicles in the ovary, the number of luteal, follicular fluid, granulosa cell layers was significantly reduced than in the control group, and with varying degrees of blood vessels to dilate. Apaf-1and Caspase-3of ovarian expressed increased (P<0.05).③111cases DOR patients were observed, the frequency of chief complaints:infertility (41.44%)> Hypomenorrhea (36.04%)> early menstruation (33.33%)> Delayed menstruation (29.73%). the frequency of symptoms:hypomenorrhea (46.85%)> Delayed menstruation (45.05%)> infertility (39.64%)> early menstruation (16.22%)> amenorrhea (7.21%)> menorrhagia (3.60%). the frequency of symptoms:Kidney Deficiency and Liver Stagnation (45.95%)> kidney deficiency and blood stasis (28.83%)> Liver-kidney yin Deficiency (8.11%)> deficiency of spleen and kidney (6.31%)> Qi stagnation and blood stasis (4.50%).④46cases were observed, after treatment,3people were cured,21were markedly effective,12were Effective,and10were invalid. Markedly effective rate was52.17%, the total effective rate was78.26%. The score of patient’s symptoms was improved60.91%after TCM than before (P<0.01). Symptoms include:menstruation, delayed menstruation, breast pain, weak waist,and so on. FSH, FSH/LH levels were significantly decreased(P <0.05).Conclus ion:①There are more smokers, and mental workers,with more consciously work pressure in diminished ovarian reserve patients.There is a strong emotional reaction, anxiety, worry, difficult to adapt to the external environment, personality factors, and also interpersonal sensitivity in DOR patients,and negative social and psychological factors such as anxiety, depression, nervousness and paranoia. Maybe that poor social-psychological factors are associated with the incidence of diminished ovarian reserve;〤hronic mental stress can lead to elevated serum FSH, reduced E2, and decrease in the number and quality of follicles in female rats, decline in ovarian reserve;〤hronic mental stress can lead to reduced the expression of Caspase-3and Apaf-lovar ian in female rats, Maybe that promote apoptosis of ovarian tissue with chronic mental stress is one of the mechanisms of leads to diminished ovarian reserve;﹉ypomenorrhea, delayed menstruation, infertility, early menstruation are the the most common clinical manifestations of DOR patients; Kidney Deficiency and Liver Stagnation, Kidney deficiency and blood stasis is a major clinical syndrome type of diminished ovarian reserve; ⑤The markedly effective rate of diminished ovarian reserve patients by TCM treatment is50.17%,and the total efficiency is78.26%. TCM treatment can also Reduce the expression of FSH and FSH/LH.