A Study On DNA Phosphorothioate Of Qinghuang Powder In Treating Myelodysplastic Syndromes

1. The study on the mechanism of Qing-Huang powder in cure MDS of Xiyuan HospitalWe gained curative effect, especially MDS. Since2000, Qing-Huang powder have treated MDS patients for a total of306cases. The total efficiency is79.5%-82.3%, remission rate is24.3%-26.5%. For instance, Professor Marou has cured55MDS patients with Qing-Huang powder and reinforcing kidney herbs,41patients (74.5%) have effectiveness. Professor Marou using Qing-Huang powder、reinforcing kidney herbs and androgen cured106MDS patients,44(41.51%)of them are excellence,53(50%) of them are in stable,9(8.49%) of them are invalid. There is non-statistic difference on treatment respond of Qing-Huang powder towards different cytogenetics. The overall effect is better than the western medicine therapy, the quality of patients life is significantly improved.2. The prospect of curative effect mechanism in treating MDSAlthough we have confirmed that the main effect of arsenic traditional Chinese medicine in the treatment of MDS is demethylation, but the mechanism of demethylation still needs further study. The problem of the differences of the concentration of arsenic in blood. Our previous study also found that the concentration of arsenic in blood has great individual difference, absorption and metabolism of arsenic in individual differences. So it is necessary to study the relationship between individual blood arsenic concentration detection and curative effect, improve clinical efficacy.The study on the phosphorothioation mechanism is a further explore based on the early stage of genetics research. It is a tries to clarify the therapeutic mechanism of Qinghuang powder.3. The study on DNA phosphorothioate of Qinghuang powder in treating MDS.It is believed that the elements of DNA:Carbon(C)、Hydrogen(H)、Oxygen(O)、 Nitrogen(N)、Phosphorus(P), are the five indispensable of the structures of the life. However, there is the sixth element--Sulfur(S).This is the phosphorothioate on phosphodiester bond of DNA. The element S substitute the O on hydroxyl group and to become phosphorothioic acid bond。 This is the first modification on the DNA structure besides the modificate on nucleic acid base. The PNAS published an article on2005--"DNA phosphorothioate is generally distribute on Bacterial Genome and quantifying".The authors are professors Lianrongwang and Shicheng. This is the importance progress in Sulfur modification on DNA--the element S incorporate in DNA structure.DNA phosphorothioate modification exists in a wide variety of bacterial species. DNA degradation is found in Streptomyces lividans the first. The chromosomes of Streptomyces lividans is degradation in electrophoresis. In research,this is result of abnormal modification, which lead to DNA double-stranded cutting reaction in electrophoresis。Resently, some researchers found this abnormal modification is DNA modification, which is related to Dnd gene clusters.In curative effect mechanism, Professor Marou found that after6months’ treatment by Qinghuang Powder, the MDS patients have only75different methylation genes with18functions by Go-analysis method, including blood coagulation, regulation of mitosis, cell-cell adhesion, protein transport, and so on. Compared to pre-treatment, the signal transduction and DNA repair have disappeared. There are21different methylation genes with8pathway by Pathway-analysis method, including Glycolysis/Gluconeogenesis, Influenza A, kidney disease, gastrointestinal disease pathways, and so on. Compared to pre-treatment, the pathways in cancer, chemokine signaling pathway, cell adhesion pathway, MAPK signaling pathway, and calcium signaling pathway have disappeared.Purpose:It’s all known that many drags like bleomycins can damage DNA; dimethylster and hydrazine can also make specificity cutting to DNA, as well as EDTA including ferriion. Qinghuang Powder, as yellow arsenic preparation, has well effect inhibition or apoptosis on cells of blood cancer. Does it affect duplex structure? Can S mix into DNA bone? Dose there exist the DNA phosphorothioate modification? They are the purpose of this study.Methods:Draw off2ml bone marrow of MDS patient, which accord with internalize standard. Chorisis and purify DNA, then prepare DNA sample of Sepharose embedding. Take advantage of BIO-RAD CHEF-DFⅢ System to pulse electrophoresis check degradation phenotype of DNA.Results:molecular weight standard samples are in M track, test specimen DNA is from N1to N10.It’s shown that DNA sample is fine, there is no DNA degradation in Tris buffer.Conclusion:Take advantage of BIO-RAD CHEF-DFⅢ System to pulse electrophoresis to analyze DNA of bone marrow in sepharose, DNA degradation has not been found, which suggest that there is no DNA phosphorothioate modification after a long time interference to patient’s marrow cells DNA bone by Qinghuang Powder.New ideas:there is no report about mammals’ DNA phosphorothioate research, neither home nor broad. This study focuses on DNA phosphorothioate modification after long-term yellow arsenic interference.