| ObjectiveTo investigate the molecular mechanism of Fructus Mume formula and its separated prescription extract on improving insulin resistance in type2diabetic rats, and to explore the potential difference of the molecular mechanism of the complicated form and of the seperated components.MethodsThe rat model of type2diabetes was established by feeding with a high-fat diet for8weeks and intravenous injection of small doses of streptozotocin. Rats in the treatment group were treated with Fructus Mume fomula and its separated prescription extract from gastric tube. The body weight before and after treatment, oral glucose tolerance test (OGTT) and insulin release test (IRT) were performed and determined. HOMA-IR was calculated. The prontein and mRNA expressions of Insr, β-arrestin-2, Irs-1, AMPK, Glut-4in liver, muscle and fat tissues were determined by Western blotting and RT-PCR respectively.ResultsCompared with the normal group, body weight increase, OGTT, IRT (Oh,1h) levels,HOMR-IR were all elevated in the diabetic rats. The protein and mRNA expressions of Insr, Irs-, AMPK, Glut-4in the liver, muscle and fat tissues were markedly decreased in diabetic rats, while the protein and mRNA expressions of P-arrestin-2in the liver, muscle tissues were aslo markedly descended in diabetic rats. Compared with the model group, body weight increase, OGTT, IRT (Oh,1h) levels, HOMR-IR were all reduced in the rats of Fructus Mume formula and its separated prescription extract groups. Meanwhile, the protein and mRNA expressions of Insr, lrs-1, AMPK, Glut-4in the liver, muscle and fat tissues were markedly increased in Fructus Mume formula and its separated prescription extract groups, and the protein and mRNA expressions of β-arrestin-2in the liver, muscle tissues were aslo markedly increased in Fructus Mume formula and its separated prescription extract groups.ConclusionFructus Mume formula and its separated prescription extract are effective on improving insulin resistence in type2diabetes rat model. Their therapeutic mechanism may be related to the increasing of the expressions of Insr, Irs-, AMPK, Glut-4in the liver, muscle and fat tissues and β-arrestin-2in the liver, muscle tissues. The significant difference of molecular mechanism between Fructus Mume formula and its separated prescription extract was not indicated in this study. The further investigation on the therapeutic mechanism of Fructus Mume formula and its separated prescription extract on improving insulin resistance is needed. |
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