The Central Activation Of Acupuncture Effects On The Yuan-Source Acupoints Of Liver And Kindy Channels To Spontaneously Hypertensive Rats (SHR)

The Yuan-source acupoints in clinical is mainly used for treatment and diagnosis of this disease from the zang-fu organs. This plays a very important role in the function of meridian acupoints. The effective and efficiency clinical results can be shown by only using or mainly using the Yuan-source acpoints combining with other different specific points formula principle. The liver channel yuan-source point-LR3(taichong) and the kindey channel yuan-soure point-the KI3(taixi)is often used as the main points or cooperate with acupuncture point to treat the hypertension. However, there is rarely complete systemic evaluation for the taichong or taixi.The past mechanism reaserch shows that acupuncture acupoints in treatment of hypertensive disease may be associated with the network of nerve-endocrine-immune mediated. However, in the past research for the part of the "central-brain" mechanism research is less. With the development of brain imaging technology, functional magnetic resonance imaging (functional magnetic resonance imaging, fMRI) and positron emission computed tomography (ct) imaging (positron emission tomography, PET), single photon emission computed tomography (ct) imaging (single photon emission computed tomography, SPECT) advanced brain imaging techniques such as more and more application in clinical and basic research. The central activation effect of acupuncture acupoints in research has entered a brand-new field. Brain neural activity needs to be associated with energy metabolism and glucose as energy material, in the brain is almost the only source of all energy of brain cells. Therefore, observation and analysis of various factors under the action of areas of the brain glucose metabolism is to know about the function of the brain to cognition. Use PET -CT technology to study acupuncture acupoints of brain activation effect, has two advantages, first, the application of PET technology to observe the effects of acupuncture on brain imaging study object; Secondly, combined with computed tomography (CT) technology can intuitively reflect the acupuncture to study brain anatomical morphology show. So this research proposed combining with PET-CT imaging technology, with "meridian specificity associated with brain" for the hypothesis that the Yuan-source acupoints this observation acupuncture liver and kidney meridian viscera disease and the treatment effect of brain imaging and the brain’s response to the specificity of the anatomical morphology on the bearing.So this research proposed by evidence-based medicine system evaluation research, clear the etheric chong, KI3, give priority to the clinical effectiveness of acupuncture treatment of hypertension. In addition, the use of PET-CT imaging technology to brain glucose metabolism to preliminarily determine acupuncture LR3, brook acupuncture point on the curative effect mechanism of hypertension treatment effect of the concentration of target area, comprehensive analysis of needle liver and kidney to the original point (LR3, brook acupuncture point) on brain function in spontaneously hypertensive rats blood pressure regulation effect rule and its possible mechanism of the central.Objective(1), using the methods of evidence-based medicine system evaluation analysis the etheric chong, KI3, give priority to the curative effect of treating hypertension evaluation, comprehensive evaluation of acupuncture LR3, brook acupuncture point effect in the treatment of hypertension, the basis for subsequent research to carry out necessary evidence-based evaluation basis.2. To investigate the acupuncture liver and kidney to the original point (LR3hole, brook acupuncture point), without going through the acupuncture point on the regulation of blood pressure in spontaneously hypertensive rats and based on PET-CT imaging technology, glucose metabolism in the brain region preliminary exploring acupuncture central activation effect of liver and kidney for approval to the original point, curative effect and mechanism of effect of the concentration of target area.Methods 1. To evidence-based medicine system evaluation method, analyse LR3, KI3mainly curative effect evaluation for the treatment of hypertension (2) in spontaneously hypertensive rats (SHR) as the animal model of experimental research in normotensive wistar rats as experimental research on animal models. Observe LR3point acupuncture, acupuncture KI3, acupuncture LR3+creek hole, acupuncture point effect on blood pressure of SHR. Combined with PET-CT technology, through with acupuncture point, SHR model group rats, and compared before and after acupuncture treatment itself. Observe effects of acupuncture LR3, KI3in spontaneously hypertensive rats area glucose metabolism, the statistical analysis of data from initial glucose metabolism in brain areas to determine the acupuncture LR3, too brook acupuncture point on the curative effect mechanism of hypertension treatment effect of the concentration of target area and to explore its possible mechanisms of inner.Results:1. Included in12RCT article, a total of1044cases. Most test quality is not high. Acupuncture versus western medicine Meta analysis showed that:(1) show the efficiency of the RR=1.80,95%CI (1.42,2.27], Z=4.93, P <0.00001, with statistical significance.2total effective RR=1.29,95%CI (1.02,1.64], Z=2.10, P=0.04, no statistical significance.(3) systolic blood pressure WMD=0.92,95%CI (4.67,2.83), Z=0.48, P=0.63, there is no statistical significance.(4) diastolic WMD=1.12,95%CI (2.15,0.08), Z=2.12, P-0.03, with statistical significance.2. Animal experiments, with the model group SHR blood pressure ratio, LR3point acupuncture group, acupuncture KI3group, the acupuncture LR3+rats after acupuncture brook acupuncture point group were blood pressure decreased, with statistical significance (P<0.05). Acupuncture the den SHR rats blood pressure and model group than blood pressure, antihypertensive effect is not obvious, no statistical significance (P>0.05). Acupuncture end state for the first time, the line immediately PET-CT scans, the SPM statistical analysis, the results showed as follows:LR3point acupuncture group SHR rats and model rats than that increased glucose metabolism of brain regions as follows:the motor cortex, the visual cortex, dorsal thalamus-the lateral nuclear group, cingulate cortex, dorsal thalamus after the medial nuclear group, cerebellum, joint the frontal cortex, sensory cortex. LR3point acupuncture group rats with acupuncture point than rats, increased glucose metabolism of brain regions as follows:the midbrain tegmentum, motor cortex, hippocampus, cerebellum anterior lobe, the periaqueductal gray, the cerebellum-after the leaf, the visual cortex. Acupuncture KI3den SHR rats with model group, higher glucose metabolism of brain regions as follows:the motor cortex, sensory cortex and striatum. Acupuncture too brook acupuncture point group rats with acupuncture point than rats, increased glucose metabolism of brain regions as follows:the olfactory bulb, auditory cortex, cerebellum anterior lobe, cingulate cortex, dorsal thalamus-lateral nucleus, hippocampus, midbrain tegmentum, motor cortex, olfactory cortex, edge forehead orbitofrontal cortex, the cortex, sensory cortex and striatum.Acupuncture LR3+KI3den SHR rats with model group, higher glucose metabolism of brain regions as follows:the amygdala, cingulate cortex, motor cortex, joint the parietal cortex, piriform cortex, front edge of the cortex, pressing of cortex, the sensory cortex, striatum, the visual cortex. Acupuncture LR3+too brook acupuncture point group rats with acupuncture point than rats, increased glucose metabolism of brain regions as follows:the cerebellum anterior lobe, cingulate cortex, dorsal thalamus nucleus lateralis, medulla oblongata, periaqueductal gray, midbrain tegmentum leading edges, motor cortex, the cortex, pressure after cortex, the visual cortex. After the acupuncture treatment, PET-CT scan line treatment, by the SPM statistical analysis, the results are as follows:Acupuncture LR3SHR rats with model group, higher glucose metabolism of brain regions:the olfactory bulb, the amygdala, cingulate cortex, motor cortex, piriform cortex, sensory cortex, dorsal thalamus the lateral nuclear group, insula, frontal cortex orbitofrontal cortex.Acupuncture LR3rats with acupuncture point group rats, increased glucose metabolism of brain regions:the motor cortex, frontal orbitofrontal cortex, the sensory cortex, the amygdala, the midbrain tegmentum, olfactory cortex, piriform cortex.Acupuncture KI3SHR rats and model group, higher glucose metabolism of brain regions:the cerebellum pompoms nodules of leaf, leaf, olfactory cortex, visual cortex after cerebellum.Acupuncture KI3group rats with acupuncture point than rats, increased glucose metabolism of brain regions:the cerebellum anterior lobe, the midbrain-inferior colliculus, midbrain tegmentum, olfactory cortex. Acupuncture LR3+KI3SHR rats and model group than, higher glucose metabolism of brain regions:the olfactory bulb, olfactory cortex.Acupuncture on LR3+KI3group rats with acupuncture point than rats, increased glucose metabolism of brain regions:the cerebellum anterior lobe, hippocampus, midbrain tegmentum, motor cortex, pons, cingulate cortex, sensory cortex.Conclusion1. Acupuncture on LR3and KI3give priority to curative effect on primary hypertension and was equal to that of western medicine, but higher quality multi-center RCT need to be confirmed.2. Acupuncture on LR3, KI3or LR3+KI3could have certain regulation effect on blood pressure in spontaneously hypertensive rats. It reduces blood pressure in spontaneously hypertensive rats.3. The use of PET-CT technology can directly reflect the situation of acupuncture and acupuncture treatment on rats by glucose metabolism. Results suggest that acupuncture on LR3, acupuncture on KI3, acupuncture on LR3+KI3on glucose metabolism in SHR regions exist differences when sitimulating the acupionts and after the acupuncture treatment. Compared with acupuncture on non-point rats, acupuncture on LR3, acupuncture on KI3, acupuncture on LR3+KI3also exist differences of glucose metabolism in SHR. Theacupuncture points-LR3, KI3has certain acupoint specificity.