Studies On The Active Components Of Cephalotaxus Lanceolata And Cephalotaxus Mannii From Yunnan Province

Cephalotaxus lanceolata and C. mannii are two characteristic plants of the genusCephalotaxus in Yunnan province, and their active componts haven′t been studiedbefore. This thesis, based on the chemical constituents from fresh leaves and branchesof C. lanceolata and C. mannii, focuses on the different ways (including desiccating,pulverizing, extracting with industrial methanol at normal temperature, merging andconcentrating the extract, examining with TLC, leaching with chloroform, aceticether and n-butyl alcohol dissolvent, laminar analysis of both normal and reversedphase, Sephadex LH-20column chromatography, means of high-efficiency liquidchromatography) used to analyze and purify these two target plants, respectively.Physicochemical constants, characters and thin layers of the standard are used tocheck with infrared inspection, ultraviolet, mass spectrum, nuclear magneticresonance spectrum along with oxidizing, restoring, hydrolyzing and derivatives,confirming the structure of compounds and conducting bioactive screen on theextracted monomeric compound with regulatory pharmacological screening mode andapproach. Totally70compounds were isolated from C. lanceolata and C. mannii,63compounds were identified by spectral techniques, including7new compounds and1natural product.41compounds have been extracted from C. lanceolata and35compounds areidentified through spectrum analysis technique (3new ones and1natural product),among which,14flavones:6-C-methyl-7,4’,4’’’-tri-O-methylamento-flavone (1),apigenin-6-C-methyl-7-O-β-D-glucopyranoside (2), amentoflavone-7,7’’,4’,4’’’-tetramethyl ether (3),7,4’,7’’-tri-O-methyl amentoflavone (4), taiwanhomoflavone A(5), ginkgetin (6),2,3-dihydro-6-methylginkgetin (7),2,3-dihydro-6-methylsequoia-flavone (8), apigenin5-O-α-L-rhamnopyranosyl-(1→3)-β-D-glucopyranoside (9),ladanetin-6-O-β-D-glucoside (10), isoscutellarein7-O-β-D-glucopyronoside (11),apigenin (12), naringenin (13),6-C-methylnaringenin (14);4alkaloids: cephalancin A(15),cephalotaxine,2,11-epoxy-1,2-dihydro-8-oxo-(2α,11α)-9(N)(16), cephalancetineB (17), drupacine (18);2internal esters:11-hydroxyhainanolidol (19), fortunolide A(20);4lignans: rel-(7α,8β)-3-methoxy-4’,7-epoxy-8,3’-oxyneolignan-4,9,9’-triol (21), [3,3’,4,4’-tetrahydro-6,6’-dimethoxy-3,3’-bi-2H-benzopyran]-4,4’-diol (22),(+)-7’(S)-hydroxyarctigenin (23),5-(3’’,4’’-dimethoxyphenyl)-3-hydorxy-3-(4’-hydroxy-3’-methoxybenzyl)-4-(hydroxymethyl)-dihydrofuran-2-one (24);5sesquiterpenes:dehydrovomifoliol (25),(6R,7E,9S)-9-hydroxy-4,7-megastigmadien-3-one (26),(6R,9R)-9-hydroxy-4-megastigmen-3-one (27), seiricardine A (28),eremopetasinorone A (29);6other components: methyl17-hydroxydocosanoate (30),sitosteryl-3β-glucoside-6’-O-palmitate (31), β-sitosterol (32), daucosterol (33),p-hydroxybenzaldehyde (34),3-methoxy-4-hydroxybenzaldehyde (35). Compounds1,2and15are new compounds, compound16is a new natural product and compounds10,11,13,21-31,34and35were isolated from this genus for the first time. Exceptfour compounds5,17,18,32and33, others were first isolated from C. lanceolata.29compounds have been extracted from C.mannii,28out of it are identifiedwith spectrum analysis technique (4new ones), there are13alkaloids: cephamanin A(36), cephamanin B (37), cephamanin C (38), cephamanin D (39), cephastigiamide A(40), deoxyharringtonine (41), drupacine (42), cephalotaxine (43), epiephalotaxine(44), cephalancetine A (45), cephalotaxine α-N-oxide (46), cephalotaxine β-N-oxide(47),3-epischellhammericine (48);4flavones: taiwanhomoflavone A (49), ginkgetin(50),2,3-dihydro-6-methylginkgetin (51), apigenin (52);1internal ester: fortunolideA (53);1diterpene:3β-hydroxy-5,6-dehydrosugiol (54);5steroidal:(5α,20R)-pregn-2-en-20-ol (55),(3β,5α,20S)-20-methylpregnane-3,21-diol (56)，(22S)-5α-ergostane-3α,22-diol (57), β-sitosterol (58), daucosterol (59);6other ingredients: ferulylaldehyde (60),[8]-dehydroshogaol (61), p-hydroxybenzaldehyde (62), methyl6-hydroxytridecanoate (63). Compounds36,37,38and39are new ones, compounds54-57and60-63were isolated from this genus for the first time, and it is also the firsttime for all compounds being isolated from C. mannii.7new compounds and1natural product extracted from C. lanceolata and C.mannii have been screened with cytotoxic activity, no inhibitory activity of in-vitrotumor is found under the condition of40μM concentration, using cultured HL-60,SMMC-7721, A-549, MCF-7and SW-480cell lines by MTT dye assay.