Levels Of Endothelial Progenitor Cells In Patients With Ischemic Cerebral Diseases And Analysis Of Related Factors

Objective: Levels of circulating endothelial progenitor cells inpatients with acute cerebral infaction and chronic cerebral ischemia weremeasured by cytometry. Levels of circulating EPC in three differentexperimental groups: acute cerebral infarction group, chronic cerebralischemia group and normal control group were compared to each other.We analyzed the correlation between level of circulating EPC in acuteischemic infarction patients and risk factors of cerebrovascular diseases.We aimed to demonstrate the meaning of EPC in the research of ischemiccerebrovascular diseases according to our research results.Method:1. Circulating EPC were isolated using staining markers:CD34+, CD133+and KDR+.4milliliter venous blood were collectedfrom25acute cerebral infarction patients,30chronic cerebral ischemiapatients to measure circulating EPC numbers. EPC level was alsoevaluated in15normal control objects. we used flow cytometry tomeasure the numbers of circulating EPC.2. Levels of circulating EPC in25acute ischemia patients were measured and their general clinical data and examination measurements were collected including systolic pressure,HbA1c and total cholesterol, triglyceride, low-density lipoprotein andhigh-density lipoprotein. We used Pearson analysis to determine thecorrelation between these risk factors and level of circulating EPC.3.Level of circulating EPC in30chronic ischemia patients were measuredand were compared among different grade of carotid artery stenosis,evaluated by digital subtraction angiography. We analyzed correlationbetween carotid intima-media thickness (CIMT), evaluated by neckvascular ultrasound, and level of circulating EPC.Result:1. Compared with normal control group, acute cerebralischemic patients and chronic cerebral ischemic patients showsignificantly fewer number of circulating EPC (P＜0.01) and acutecerebral ischemic patients have lower level of EPC than chronic cerebralischemic patients (P＜0.01).2. Level of EPC in acute cerebral infarctionpatients is in a moderate negative correlation with systolic pressure, totalcholesterol and low-density lipoprotein (P＜0.05) and is in a mildnegative correlation with HbA1c and triglyceride (P＜0.05).3. Comparedwith normal control group, levels of circulating EPC in mild andmoderate-severe carotid artery stenosis patients decrease (P＜0.05) andmoderate-severe stenosis patients have lower level of EPC than mildstenosis patients (P＜0.05). CIMT in chronic ischemia patients is in amoderate negative correlation with level of circulating EPC (P＜0.05). Conclusion: EPC in acute cerebral infarction patients and chroniccerebral ischemic patients decrease obviously. Decrease in number ofEPC is one of the risk factors of cerebral ischemia. Level of EPC hasstrong correlation with cerebrovascular disease risk factors bloodpressure, total cholesterol, low-density lipoprotein and carotid arterystenosis. EPC is of significant clinical value in the prediction, preventionand treatment of cerebral ischemia diseases.