Experimental Study Of Rheumatoid Arthritis Based On Flow Cytometry

Object:Establishment of peripheral blood T lymphocyte subsets TCR detection methods, clinical significance TCR Subsets and explore traditional Chinese medicine may be effective mechanisms of action on this basis.Method:According to the antigen-the basic principles of immunology antibody binds specifically, the use of multiple fluorescent antibody staining, flow cytometry analysis by photoelectric signal strength CD3, CD4, CD8, CD95, CD69, TCR, Annex Inv antibodies and other signs, establish gating strategy, peripheral blood lymphocytes were detected and analyzed. Through different clinical groups, the method detected the clinical significance of indicators discussed. On the basis of efficacy indeed possible mechanism of Chinese medicine research.Result:1Normal population of peripheral blood T lymphocyte subsets TCR proportion of cells is different, but each group of cells can be detected.2Normal population in peripheral blood, the expression of molecules Annex Inv T lymphocytes CD95+CD69+T the highest proportion.3Patients with rheumatoid arthritis, peripheral blood T lymphocyte subsets in TCR Vβ12, Vβ20and CD4+Vβ9cell subsets proportion significantly different compared with the normal population (P<0.05), compared with patients with systemic lupus erythematosus CD8+Vβ14, C84+Vβ4proportion of significant differences compared to the normal population (P<0.05); peripheral blood T lymphocytes, CD95+T cells, CD69+no significant difference compared with the proportion of T cells in the normal population (P>0.05), compared with patients with systemic lupus erythematosus significant difference (P>0.05). 4Outside of rheumatoid arthritis patients in different stages of the course week TCR T lymphocyte subsets in the blood, the proportion of Vβ12subpopulations significant differences (P<0.05).5Before and after the traditional Chinese medicine treatment of rheumatoid arthritis patients with changes in the proportion of subsets of T lymphocytes TCR subsets, Vβ7.1, Vβ18, Vβ8, Vβ14and other significant differences (P<0.05); peripheral blood lymphocytes, Annex Inv molecule expression in T lymphocytes CD95+CD69+T proportion of CD95+T cells and the percentage change in the expression of cellular molecules Annex Inv significant difference (P<0.05).Conclusion:1Normal population in peripheral blood T lymphocyte subsets were distributed TCR.2The normal population, apoptosis induced Fas pathway is a major mechanism of apoptosis of T lymphocytes.3Different diseases, different distributions of peripheral blood T lymphocyte subsets TCR, Vβ12, Vβ20, CD4+Vβ9rheumatoid arthritis may occur in specific cell subsets relative.4Different stages of the course of rheumatoid arthritis, a different distribution of peripheral blood T lymphocyte subsets TCRVβ12and CD4+Vβ9proportional change in the peripheral blood of RA remission can be used as indicators to judge the situation.5Traditional Chinese medicine may increase the number of CD95+T cells, reducing T cells abnormal increase of CD69, Fas pathway activation in lymphocytes and other ways to adjust the proportion of apoptotic cells, changing the distribution of TCR subsets, so as to achieve the treatment of rheumatoid arthritis purposes. Chinese herbal compound may have a two-way adjustment.