Based On Th1/Th2, MMPs/TIMPs To Research The Influence Of Fire Needle On Sihua Acupoint In Chemotherapy Patients With None-small Cell Lung Cancer

ObjectiveIn this study, a randomized controlled clinical study was used to observe the clinical effect, quality of life and the impact of anti-cancer drug toxicity of fire needle on Sihua acupoints (Geshu BL17, Danshu BL19) in chemotherapy patients with non-small cell lung cancer. The level of T cell subsets, NK cells, B cells, and IFN-γ, IL-4, MMP-9, TIMP-1 were detected in chemotherapy patients with non-small cell lung cancer. We diacussed the possible mechanism that fire needle on Sihua acupoints produced to influence the clinical effect, quality of life and the impact of anti-cancer drug toxicity in chemotherapy patients with non-small cell lung cancer through regulating the balance between Thl and Th2, the balance between MMP-9 and TIMP-1.MethodsIn this study, non-small cell lung cancer patients(n=60) were randomly divided into observation group and control group. Patients in the observation group the observation group were treared with fire needle on Sihua acupoints combined with chemotherapy. While Patients in the control group were treared only with chemotherapy. In the control group, patients were treated with four kinds of chemotherapy which were TP (paclitaxel plus cisplatin)/GP (gemcitabine plus cisplatin)/DP (docetaxel plus cisplatin)/NP (vinorelbine plus cisplatin). When patients in the observation group treating with TP/ GP/DP/NP chemotherapy, fire needle on Sihua acupoints was given once a day for 7 days with a course of treatment at the same time. After treatment RECIST criteria was used to evaluate clinical effect. Before and after treatment, Karnofsky Performance Status and Functional Assessment of Cancer Therapy-lung were used to evaluated quality of life, acute and subacute toxicity of anti-cancer drugs indexing table was used to make Anti-cancer toxicity evaluation. Before and after treatment,the level of T cell subsets (total T cells, Th cells, Th cells, Th/Ts), NK cells, B cells Flow cytometry in Venous blood of non-small cell lung cancer patients were detected by Flow cytometry. the level of IFN-γ, IL-4, MMP-9, TIMP-1 in serum were detected by by enzyme-linked immunosorbent assay (ELISA).ResultsComparison of RECIST evaluation:In observation group, the tumor response rate is 20.00%, the disease control rate is 73.33%. In observation group, the tumor response rate is 16.67%, the disease control rate is 63.33%. Compared with the control group, the tumor response rate and the disease control rate are higher in the observation group (fire needle combined with chemotherapy), but there were not statistically significant differences between two groups in both the tumor response rate and the disease control rate.Comparison of the quality of life; After treatment, KPS score is higher in the observation group than in observation group, there were statistically significant between two group. In observation group, KPS score is higher after treatment than before treatment, but there were no statistically significant differences between two groups. After treatment, FACT-L QoL scores, daily field activities, social/family situation, emotional status, activity areas of capacity and the score of other factors in observation group were higher compared with control group. In the daily activities, the field of activity, other factors areas and FACT-L QoL scores there were statistically significant differences between the two groups, but there were no statistically significant differences between the two groups in social/family situation and emotional status.Comparison of acute and subacute toxicity of anti-cancer drugs:The acute and subacute toxicity of anti-cancer drugs in blood system in observation group:degree I was 2 cases (6.67%), degree Ⅱwas 3 cases (10%), degree Ⅲ was 2 cases (6.67%), degree Ⅳ was 1 case (3.33%). In control group, degree Ⅰ was 1 cases (3.33%), degree Ⅱ was 7 cases (23.33%), degree Ⅲ was 3 cases (10%), degree Ⅳ was 1 case (3.33%). There were statistically significant differences between the two groups in blood system and hemoglobin, white blood cell, neutrophil and platelet. The acute and subacute toxicity of anti-cancer drugs in the gastrointestinal tract:degree I was 2 cases (6.67%), degree II was 3 cases (10%), degree Ⅲ was 2 cases (6.67%), degree Ⅳ was 1 case (3.33%), and there were statistically significant differences between the two groups.Comparison of T cell subsets, NK cells, B cells:After treatment, compared with control group, total T cells、Th/Ts are higher in observation group, and there were statistically significant between the two groups. After treatment, Th cells was higher in observation group compared with control group, and there was no statistically significant between the two groups. After treatment, compared with control group, Ts cells is lower in observation group, and there was statistically significant between the two groups. After treatment, NK cells, B cells were higher in observation group compared with control group, and there were statistically significant between the two groups.Comparison of IFN-γ IL-4:Before treatment, the level of IFN-Y, IL-4 were 15.21 ± 3.50pg/ml and 39.30 ± 5.07 pg/ml in observation group. After treatment, the level of IFN-γ, IL-4 were 19.02±3.51 pg/ml、29.45±3.14 pg/ml in observation group. Before treatment, the level of IFN-γ,IL-4 were 15.49 ±3.43pg/ml、38.61±4.30 pg/ml in control group. After treatment, the level of IFN-γ, IL-4 in were 12.34±2.95pg/ml、50.74±4.47 pg/ml in control group. After treatment, compared with control group the levels of IFN-γ was higher in observation group, the levels of IL-4 was lower. And here were statistically significant between the two groups in both of them.Comparison of MMP-9、TIMP-1、MMP-9/TIMP-1:Before treatment, the level of MMP-9、TIMP-1、MMP-9/TIMP-1 were 755.16±48.06pg/ml、621.26±91.11 pg/ml、 1.24±0.20 in observation group. After treatment, the level of MMP-9、TIMP-1、 MMP-9/TIMP-1 were 383.14±61.10pg/ml、359.34±43.43 pg/ml、1.07±0.13 in observation group. Before treatment, the level of MP-9、TIMP-1、MMP-9/TIMP-1 were 776.60±56.17pg/ml、620.74±56.03 pg/ml、1.26±0.11 in control group. After treatment, the level of MMP-9、TIMP-1 in were 445.05±44.77pg/ml、 387.77±58.46 pg/ml、1.16±0.82 in control group. After treatment, compared with control group the levels of MMP-9、TIMP-1、MMP-9/TIMP-1 were higher in observation group. And there were statistically significant between the two groups in both of them.ConelusionFire needle on Sihua acupoints could make a certain improvement on clinical effection and also on quality of life, especially on daily field activities, activity areas of capacity and the score of other factors in chemotherapy patients with non-small cell lung cancer. And it can reduce the incidence of blood system and the gastrointestinal system in acute and subacute toxicity of anti-cancer drugs. Fire needle on Sihua acupoints can increase the level of IFN-Y in serum and reduce the level of IFN-Y in chemotherapy patients with non-small cell lung cancer. Through this.it can regulate the Th1/Th2 balance to inhibit the Th1/Th2 shift. In chemotherapy patients with non-small cell lung cancer, while fire needle on Sihua acupoints could reduce the level of IMMP-9, TIMP-1 to regulate the balance of MMPs/TIMPs to inhibite tumor invasion and metastasis.