The Significance And Relationship Between The Expression Of Cripto-1and β-catenin In Endometriosis And Adenomyosis

Objective:To investigate the expression and relationship between Cripto-1and (3-catenin in paired ectopic and eutopic endometrium of ovarian endometriomas, adenomyosis and ovarian endometriomas concomitant with adenomyosis patients, so as to explore their roles in the pathogenesis of ectopic focus progression.Methods:To detect the expression profile of Cripto-1and β-catenin protein in paired ectopic and eutopic endometrium of45patients with ovarian endometriomas (Chocolate cyst group),45patients with adenomyosis (Adenomyosis group),45patients with ovarian endometriomas concomitant with adenomyosis (Chocolate cyst combined with adenomyosis group) and45cases of normal endometrium (control group) by Immunohistochemistry.Results:1.The positive ratio of Cripto-1protein expression was no significantly differences in ectopic and eutopic endometrium of Chocolate cyst group、Adenomyosis group and Chocolate cyst combined with adenomyosis group (P>0.05), and up-regulated significantly in comparison of control group (P<0.05). The positive ratio of Cripto-1protein was46.67%for ectopic endometrium of Chocolate cyst group,53.33%for ectopic endometrium of Adenomyosis group,57.78%for ectopic endometrium of chocolate cyst in Chocolate cyst combined with adenomyosis group and66.67%for ectopic endometrium of adenomyosis in Chocolate cyst combined with adenomyosis group, which is similar between the two groups (P>0.05). The positive ratios of the above three groups were62.22%、64.44%and75.56%, which is no significantly difference between groups (P>0.05) and significantly higher than the positive ratio of control group (P<0.05). The positive ratio of stage III-IV in ectopic endometrium of Chocolate cyst group were higher than that of stage Ⅰ～Ⅱ with significance (P<0.05). Meanwhile the positive ratio of stage III-IV in ectopic endometrium of chocolate cyst of Chocolate cyst combined with adenomyosis group were significantly higher than that of stage Ⅰ～Ⅱ (P<0.05).2.The positive ratio of β-catenin protein expression was significantly different in paired ectopic and eutopic endometrium between Chocolate cyst group and Adenomyosis group (P<0.05), and down-regulated significantly in comparison of that of control group (86.67%). The positive ratio of β-catenin protein expression in ectopic endometrium of chocolate cyst and ectopic endometrium of adenomyosis in Chocolate cyst combined with adenomyosis group was significantly different versus paired eutopic endometrium (P<0.05), and decreased significantly in comparison of control group (P<0.05). The positive ratio of β-catenin protein was31.11%for ectopic endometrium of Chocolate cyst group,53.33%24.44%for ectopic endometrium of Adenomyosis group,26.67%for ectopic endometrium of chocolate cyst of Chocolate cyst combined with adenomyosis group and17.78%for ectopic endometrium of adenomyosis of Chocolate cyst combined with adenomyosis group, which is no significantly differences between groups (P>0.05). The positive ratios of the above three groups were62.22%、57.78%and66.67%, which is also no significantly differences between groups (P>0.05)and significantly lower than the positive ratio of control group (P<0.05). The positive ratio of stage III-IV in ectopic endometrium of Chocolate cyst group were lower than that of stage Ⅰ～Ⅱ with significance (P<0.05). Meanwhile the positive ratio of stage Ⅲ～Ⅳ in ectopic endometrium of chocolate cyst of Chocolate cyst combined with adenomyosis group were lower than that of stage Ⅰ～Ⅱ with significance (P <0.05).The cytoplasm positive ratio of P-catenin protein expression was significantly different between the paried ectopic and eutopic endometrium among the Chocolate cyst group, Adenomyosis group and Chocolate cyst combined with adenomyosis group, comparing with that of control group (P<0.05).3.The Spearman analysis showed that negative correlations between Cripto-1protein and β-catenin protein in paired ectopic and eutopic endometrium of the Chocolate cyst group, Adenomyosis group and Chocolate cyst combined with adenomyosis group(P<0.05).Conclusion:1.The expression of Cripto-1protein was significantly up-regulated in eutopic endometrium of endometriosis and adenomyosis versus the normal endometrium while β-catenin protein was significantly down-regulated, which indicated that they might be involved in the pathogenesis of endometriosis and adenomyosis.2.The target site of Cripto-1protein and β-catenin protein was the eutopic endometrium, which suggested that the two proteins were related with the mechanism of migration, invasion and plantation of eutopic endometrial cells.3.The expression of Cripto-1protein and β-catenin protein were relevant with different clinical stages of endometriosis, which indicated that they might be a promising indicator of assessing the disease severity and progression.4.There was a negative relationship between the Cripto-1protein and β-catenin protein in paired ectopic and eutopic endometrium of endometriosis and adenomyosis, which suggested that they may involved in the development of ectopic focus progression.