Tyrosine Phosphorylation And Protein Expression Of Insulin Receptor Substrate2in Ovarian Luteinizing Granulose Cells From The Patients Of Polycystic Ovary Syndrome With Phlegm-dampness

Objective:1. To study the distribution regularity of syndrome essential elements of Polycysticovarian syndrome based on literature data.2. To detecte the degree of tyrosine phosphorylation and protein expression of insulinreceptor substrate2in ovarian luteinizing granulose cells from the patients of polycysticovary syndrome with Phlegm-dampness, and discuss the molecular mechanism of insulinresistance and the pathogenesis of reproductive dysfunction in the patients of polycysticovary syndrome with Phlegm-dampness.Methods:1. Literatures about PCOS collected from CBM,CNKI and TCM, the criterion and thedatabase of TCM syndrome of PCOS were setup, SPSS16.0for windows software wasadopted for statistical description.2. Select60PCOS patients under treatment of vitro fertilisation and embryotransplantation （IVF-ET）（30cases in phlegm-dampness group,and30cases innon-phlegm-dampness group） and30cases oviduct infertility patients （control group）promote ovarian syndrome after superovulation granular cell. Use of steady-state model（homeostasis model assessment, HOMA） to calculate insulin resistance index（HOMA-IR）; By immune imprinting（Western blot）method to detect degree of IRS-2syndrome protein expression and tyrosine phosphorylation of ovarian granulosa cell in thethree groups.Results:1. In all the syndrome factors, there was5disease location ones and9disease nature ones. In the5disease location syndrome factors, kidney had the highest frequency74.03%.The main pathological deficiency factors of PCOS was yang deficiency with the frequency67.53%. The main pathological overabundance factors of PCOS was dampness with thefrequency67.53%. In the combination of essential elements of syndrome, the combinationof two（44.16%）and three（33.77%） elements have higher frequency. And the othercombination was relatively few. The combination of essential elements of syndrome withdeficiency factors and overabundance factors had the highest frequency72.73%.2.Comparison of the protein expression of granular cell IRS-2in each group:Compared with control group, the protein expression of IRS-2in two groups of PCOSpatients were significantly higher （P <0.01）, and PCOS phlegm-dampness group wassignificantly lower than non-phlegm-dampness group （P <0.05）.3. comparison of degree of IRS-2tyrosine phosphorylation in granular cell: Comparedwith control group, IRS-2tyrosine in two groups of PCOS were significantly lower （P <0.01）, and PCOS phlegm-dampness group was significantly lower than non-phlegm-dampness group （P <0.01）.Conclusions:1.So the main syndrome target point viscera is the kidney. The main pathologicalfactors of PCOS was dampness and they often combine each other.2.Local area of ovarian in patients of phlegm-dampness PCOS group has insulinresistance, its reason may related with anomalies of protein expression of IRS-2andtyrosine phosphorylation，provide objective basis for the diagnosis of PCOS phlegm-dampness syndrome.3.“Tan Shi”is the main important aspects in format the insulin resistance of PCOSphlegm-dampness.