Study On Biological Index Of Erythrocyte Membrane Of Three Activating Microcirculation And Removing Stasis Prescription To Qi Stagnation And Blood Stasis Model Rats

Objective:Using3formulas for activating blood circulation as probe, Qi-stagnation and blood stasis model as research object, and bioindicator of erythrocyte membrane as target, to discuss the effect of three formulas on bioindicator of erythrocyte membrane of Qi-stagnation and blood stasis model. In order to clear syndrome essence of Qi-stagnation and blood stasis model, and reveal action mechanism of3formulas at the molecular biology level of erythrocyte membrane.Methods:Rats were used as research objects. We used the method of unpredictable stimulation with adrenaline subcutaneous injection to copy Qi-stagnation and blood stasis model of rats. At the same time, rats received gastric gavage of Buyang Huanwu Decoction, Danshen Decoction, and Shaofu Zhuyu Decoction with high and low dose. Constant stimulation and drug administration for two weeks until rats showed characterization of Qi-stagnation and blood stasis. We took blood from carotid artery, and detected whole blood viscosity, plasma viscosity, blood sedimentation K value, erythrocyte deforma-bility, four coagulation tests, TC and TG content. We took blood to prepare erythrocyte membrane, and detected erythrocyte membrane sialic acid, sulfydryl and content of membrane SOD, MDA, cholesterol. We tested Na+-K+-ATP enzyme activity membrane; Using SDS-PAGE and SYNGENE to detect the composition and content of the membrane protein.Results:Compared with blank group, whole blood viscosity, plasma viscosity, blood sedimentation K value, content of serum cholesterol (CHO) and FIB in Qi-stagnation and blood stasis model group increased significantly, and HBX, triglyceride (TG) decreased significantly, APTT and PT shortened significantly, erythrocyte membrane MDA and cholesterol increased signifi-cantly (P<0.05, P<0.01).Compared with model group, three formulas of Buyang Huanwu Decoction, Danshen Decoction, and Shaofu Zhuyu Decoction all could increase HBX of Qi-stagnation and blood stasis model under low and high shear rate significantly, and prolong APTT significantly (P<0.05, P<0.01), increase Na+-K+-ATP enzyme activity on erythrocyte membrane significantly (P<0.05); Three formulas all could decrease blood sedimentation K value (P<0.05) and content of FIB significantly (P<0.05, P<0.01). Shaofu Zhuyu Decoction and Danshen Decoction all could significantly reduce blood viscosity and membrane cholesterol (P<0.05, P<0.01). Buyang Huanwu Decoction, Danshen Decoction could significantly increase HBX under low and high shear rate, and significantly reduce plasma CHO and MDA content on membrane (P<0.05, P<0.01). Danshen Decoction could significantly reduce whole blood viscosity under middle and low shear rate (P<0.05). Buyang Huanwu Decoction could significantly prolong PT, and significantly shorten TT (P<0.05).Compared with model group, Buyang Huanwu Decoction could significantly reduce the content of band4.2protein, while significantly increase the content of band6protein (P<0.05). Shaofu Zhuyu Decoction could significantly reduce the content of band4.2protein, while significantly increase the content of band5protein and the original ball regulatory proteins (P<0.05, P<0.01). Danshen Decoction could significantly increase the content of band4.1protein (P<0.05).Conclusion:1. Macroscopic hemorheology indexes such as whole blood viscosity, plasma viscosity, HBX, blood sedimentation K value, FIB in model group all showed significant changes and measured up to diagnostic criteria of blood stasis syndrome.2. Three formulas of Buyang Huanwu Decoction, Danshen Decoction, and Shaofu Zhuyu Decoction all could improve erythrocyte deformation ability of Qi-stagnation and blood stasis model; They could increase Na+-K+-ATP enzyme activity on erythrocyte membrane, prolong APTT, reduce the content of FIB and reduce high blood coagulation state; They could reduce blood sedimentation K value, and prevent erythrocyte aggregation. Danshen Decoction was better than other two formulas in aspects of raising erythrocyte deformation ability, reducing the content of FIB and prolonging APTT. 3. Shaofu Zhuyu Decoction and Danshen Decoction could correct high blood viscosity and high membrane cholesterol content in Qi-stagnation and blood stasis model group; Buyang Huanwu Decoction and Danshen Decoction could decrease the plasma CHO content and MDA content on membrane; In terms of reducing plasma CHO and membrane cholesterol, Danshen Decoction was better than that of the other two. Danshen Decoction could improve whole blood viscosity increasing of Qi-stagnation and blood stasis model; Buyang Huanwu Decoction could significantly prolong PT and significantly shorten the TT.4. Buyang Huanwu Decoction could act on erythrocyte membrane band4.2protein and band6protein; Shaofu Zhuyu Decoction could act on the original ball regulatory proteins, band4.2protein and band5protein; Danshen Decoction could act on band4.1protein.