MicroRNA-107 Functions As A Candidate Tumor Suppressor Genein Clear Cell Renal Cell Carcinoma Involving Several Genes

Background:MicroRNAs (miRNAs) are small RNAs with oncogenic and tumor-suppressing roles in cancer; miRNAs not only regulate various target genes but also participate in vital signaling pathways.Methods and results:The tumor suppressor role of miR-107 was confirmed in clear cell renal cell carcinoma (ccRCC) in 52 paired clinical specimens and renal cell carcinoma cell lines. Significant correlations were noted between clinical features and miR-107 expression level. Lentiviral vector and biosynthesis mimics were used to overexpress pre-miR-107 or mock miR-107 to further investigate the tumorigenesis of miR-107 in vivo and in vitro. Cell proliferation and metastasis were inhibited in 786-0 cell line after miR-107 was delivered. High miR-107 level can induce cell cycle arrest at G2/M phase and retard tumor growth in nude mice apparently. In addition, eukaryotic translation initiation factor 5 (EIF5) was demonstrated as a direct target of miR-107 and had an inverse relationship with miR-107. p53 and VHL genes which are implicated in renal tumor, were associated with miR-107.Conclusion:In summary, our results suggested that miR-107 can inhibit cell proliferation and migration of renal cell carcinoma; furthermore, this study may provide a potential therapeutic regime of ccRCC treatment...