The Study On The Role Of The Hsa_circ₀008344 In Glioblastomas Progression By Using The Circular RNA And Tissue Microarray

Objective:In this study,we constructed tissue microarray and applied circular-RNA microarray to investigate the difference between glioblastoma and normal brain and observed a novel circ-RNA,hsa_circ₀008344,overexpressed in glioblastoma.Knockdown of hsa_circ₀008344 inhibited glioblastoma cell prolif-eration,migration,and invasion,and increased cell apoptosis,suggesting that hsa_circ₀008344 might play an oncogenic role in the development and progression of glioblastoma.Methods 1)the construction of glioam tissue microarray;2)the construction of the circular RNA micriarray.3)Human circ RNA microarray analysis was performed to screen the expression profile of circ RNAs in IDH1 wild-type glioblastoma tissue.4)The expression of hsa_circ₀008344 in glioblastoma and normal brain samples was quantified by q RT-PCR.5)Functional experiments were performed to investigate the biological functions of hsa_circ₀008344,including MTT assay,colony formation assay,transwell assay,and cell apoptosis assay.Results Circ RNA microarray revealed a total of 417 abnormally expressed circ RNAs?>1.5-fold,P <.05?in glioblastoma tissue compared with the adjacent normal brain.Hsa_circ₀008344,among the top differentially expressed circ RNAs,was significantly upregulated in IDH1 wild-type glioblastoma.Further in vitro studies showed that knockdown of hsa_circ₀008344 suppressed glioblastoma cell proliferation,colony formation,migration,and invasion,but increased cell apoptotic rate.Conclusions Hsa_circ₀008344 is upregulated in glioblastoma and may contribute to the progression of this malignancy.