| An instrument system capable of concurrent imaging of surfaces by surface plasmon resonance microscopy (SPRM) and surface plasmon resonance-enhanced fluorescence (SPRF) is presented. A conventional laser pointer is adopted as a light source, and a reflective diffuser coupled to an acoustic transducer is used to remove speckle artifacts due to coherent beam interference. Both SPRM and SPRF systems are characterized, and a careful choice of widely available, inexpensive, off-the-shelf components allows the entire system to be constructed at low cost. A model streptavidin-biotin system is explored utilizing the different modalities of the instrument.;Applications of the system include mobile, cost-effective point-of-care diagnostics system and research laboratories in resource-limited settings where cost efficacy is a prime concern. |
PDF Full Text Request