| he kinetics and mechanisms of iron removal from the iron transport protein, human serum transferrin, are explored using physical inorganic techniques.;Iron removal from transferrin by the synthetic catechol sequestering agent, 1,5,10-N,N;The kinetics of iron release from the ferric transferrins by several types of ligands are carried out to discriminate between proposed mechanisms. The maximum observed rates are dependent on ligand type, indicating that the dissociation of an iron/transferrin/ligand complex is rate-limiting. Mediators which facilitate iron release operate by different pathways.;The first spectroelectrochemical measurement of the formal reduction potential of iron transferrin is carried out using methyl viologen to mediate electron transfer to the protein. A high ionic strength is necessary to effect reduction, supporting the observation that ions play an important role in the reduction of iron in transferrin.;Electrophoretically purified... |
