Genomic Library Construction, Random Sequencing, And Gap And SufC Function Of Halomonas Meridiana From Deep-sea

Halomonas meridiana, surviving in extreme environment, was a novel specie first isolated by James etc from the salt lake in Antarctic in 1990. Information regarding to classification and development of extreme enzymes is available now, yet few studies are involved in the genomics and proteomics for this bacteria.Firstly, the proteomics methodologies were applied to characterize the outer membrane proteins of H. meridiana. We failed to obtain the exact identification of the differential proteins because of the absence of genomic sequence information for H. meridiana or its relatives.To overcome the shortcoming by proteomics approach mentioned above, we constructed the genomic library of H.meridiana DSM5425, randomly sequenced 100 clones, and analyzed their homologies. Then, the ORF of three genes were fully sequenced, cloned, in which gap and sufC were expressed for antibody preparing. Furthermore, more attention is paid to the gap for its enzyme dynamic, and some researches are made for the sufC about its expression level in different culture conditions.Our work will be a valuable reference to the development and research of deep-sea microbes and a positive push to the study at the molecular level of these kinds of bacteria.