Analysis And Chromosomal Localization Of Insulin-like Growth Factor (IGF-I) Gene Of Mongolia Horse

Insulin-like growth factor-I is a multifunctional regulator of cell proliferation, which is a 70 amino acid peptide alkaline, mainly synthetized by the liver, the majority of it is in blood in the form of the binding protein. It has functions that promotes protein deposition, ossature growth, and restricts fatty tissue development.This study extracted total RNA from fresh liver tissue of the Mongolia horse, sequenced IGF-I cDNA by RT-PCR technology and compared Mongolia horse IGF-I gene coding sequence with other mammals. The result showed the homology of Mongolia horse IGF-I gene coding sequence with cattle, sheep, pigs, dogs, people were 94.2%, 93.1%, 95.2%, 81.8% and 83.5% respectively and showed that the sequence IGF-I gene coding is very conservative in molecular evolutionary. Designed specific upstream primer according to the known partial cDNA, through RACE-PCR, cloning, sequencing, obtained Mongolia horse IGF-I cDNA 3 'end sequence. According to the known sequences of cDNA designed the probe, this study positioned horse's IGF-I gene on the 28th chromosome by fluorescence gene positioning technology and with the use of electronic positioning technology.