Studies On The Morphological Structure And The P63 Protein, β-catenin, Substance P's Expression In Skin Sense Receptor Of Phrynocephalus Frontal

The experiment studied on the morphological structure and proteins' expression in the skin and integument appendage-sense receptor of Phrynocephalus frontalis to speculate the function of sense receptor in development and evolution,and the homologous relationship between the feathers and skin sense receptor.The experiment used the structural dye- HE dyeing method to invest the the morphological structure of skin and skin sense receptor in epidermis of Phrynocephalus frontalis.Through the immunochemistry method targeted the three kinds of keratins.The results indicated(1) the skin of the Phrynocephalus frontalis compose of epidermis and dermis. There was a especial structure- skin sense receptor,The skin sense receptors are cap-like protubulances residing in the scale pits,the surface of which takes on disk structure is covered with a long,thick sense hair.(2) About 21%of the scales of Phrynocephalus frontalis have the skin sense receptors and usually one scale has only one receptor. However,we find that some scales of head possessed 2-3 sense receptors, which indicated the important part of head in sensing outside stimuli. (3) The general epidermis compose of five layers which were oberhautchen, corneousβ-layer,mesos,corneousα-layer and germianal layer from outer to inner.The structure of epidermis of sense receptor was resemble that of general epidermis,but the corneousβ-layer become thinner. There was a Meissner's corpuscle- like structure in the dermis of sense receptor,which adjact to nerve fiber.We speculated the function of sense receptor may feel temperature and machine stimulation,sensitive to radiant heat energy.It may regulate the body's temperature.(4) Compare with the distributions of different types of reptilian sense receptor, we found that the living environment can influence the distribution of the sense receptor.(5) We also located the p63 protein,β-catenin, Substance P through the immunohistochemistry method,which show that stem cells of integument appendage of Phrynocephalus frontalis distribute in the germinal layer.Compared with the location of feather stem cells,can further study on the homology relationship between reptilian integument appendage and feather of bird.