Auxin And Cytokinin Regulate Organ Regeneration Of Arabidopsis In Vitro

In vitro organ regeneration is important in the study of plant development. Many factors including hormone is crucial for the organ regeneration In vitro. However, little has been done to analyze systematically on these factors yet. In this paper, a one-step organ regeneration system was constructed, using the rosette leaves of Arabidopsis as explants. We analyzed the effects of several factors on the organ regeneration in vitro.such as the concentration and species of hormones, the ecotypes and organ resources of explants.Whether the concentration of auxin, or the concentration of cytokinin is fixed, the frequency of the organ regeneration is correlated with the concentration of the other hormone, that is, the frequency of the organ regeneration increases at the relative lower concentration, however, when the concentration continued to be enhanced, the regeneration frequency decreases dramaticly.NAA are more efficient to regenerate organs than 2,4-D and IAA. The root regenerated by NAA is better than the others. The formation of organs is more efficient on medium containing zeatin than 6-BA. In a word, different kinds of hormone result in different organ regeneration.The higher cytokinin to auxin ratio is easy to regenerate shoot, when the lower regenerate root. The frequencies of shoot regeneration from different ecotypes of Arabidopsis are different. The shoot regeneration from explants of Ws (WassileWskija) ecotype is more efficient than the Col (Columbia) ecotype. Different organ resources influence the organ regeneration in vitro. Root is easier for the organ regeneration than rosettle leaf.Our results indicate that both the homone and explant are crucial for the organ regeneration, which might be informative for the research of hormone-regulated organ regeneration In vitro.