| Arbitrary discharge phenolic wastewater caused the water resource deterioration, which has a torturing influence on people's health and their living quality, and it has become very important limitary factors for urban sustainable development. So we must take effective measures to deal with phenolic wastewater and improve water environment. Although at present there are more research about the biological treatment of phenolic wastewater, but the effect of treatment of high concentration phenolic wastewater are not very satisfactory. When treating with high concentration phenolic wastewater, phenol consistency must be recede through dilution before inlet,some diluted times can reach a dozen times, make the treatment cost increased substantially. If you can increase the microorganisms degree of tolerance of phenol and reduce the diluted times, it has more difference significance for reduction of phenolic wastewater treatment cost.Screening seven strains of psychrotolerant bacterias to better phenol degradation at low temperature (10℃) in Harbin Taiping Wastewater Treatment Plant, which can used phenol as the only available carbon source and nitrogen source. In the experiment, the morphology, environmental factors, medium composition, inoculation, substrate diversity perspective of physiological ecology of these seven bacterial degradation characteristics are studied. The optimal conditions of phenol degradation at low temperature was 160 r/min, 10℃, pH 7.5, inoculation was 10% (V/V). The seven strains of phenol have some adaptability, when the phenol concentration in the 0.2 g / L , the degradation rate of over 80%, individual strains and even reached 100%. Phenol concentration is 0.5 g/L-1.0 g/L, the degradation rate of more than 90%, the largest concentration of phenol degradation of 3 g/L. Glucose and trace elements can contribute to strains on the degradation of phenol. When the initial concentration of certain bacteria was fixed, the degradation rate of phenol was related to phenol initial concentration of the principal.The analysis results of bacteria DGGE map show that the system of microbial has diversity of population. The brightness of the band show that these bacteria are dominant fungi in all systems. The groups of bacteria in water environment have strong activity. 2, 4, 24, 28, bands are diversity, these fungi show strong activity in sewage system .Furthermore,we have got 4 plants of ray fungi and 6 plants of microzyme which could regular growing in containing phenol or low temperature environment. We have did thorough knowledge of physiologic characteristic of these ray fungi and microzyme respectively. At the same time we study on morphological appraisement and degradation characteristics of microzyme with the effect of different environmental factors.Influence of psychrophile on degradation rate of phenol in sewageinin the northeast is analysised from molecular point view, and according to the regulation mechanism of psychrophile, identification of phenol degradation psychrophil effect mechanism of sewage treatment. In the research method, the basic technique of microbiology, combining the genetics and biochemistry analysis method, first separate actinomyces and yeast which can grow in low temperature to 10 degrees, and they can degradated of phenol andbroke the conventional temperature on the cultivation actinomyces and yeast. The first separation of degradation phenol fungal at low temperature yeast, research six strains yeast through physiological and biochemical mechanism and degradation characteristics, we also identified the species of six strains yeast.Low temperature and highly-efficient phenol degradation strain has a long generation time,impeded by it's physiologic characteristic and many other ecological factor . With thorough knowledge of physiological ecology, we have a try to carry on technological innovation in cold city waste water treatment. It could provide technical support for Northeastern rust belt. We could provide reference data plan to lay the first stone to process wastewater which are containing compact, efficient and fast.
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