| Resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (RNLS) such as double frequency scattering (DPS) and second order scattering (SOS) etc. are new technologies developed in 1990s. For its remarkable characteristics of high sensitivity, simple operation and good selectivity, this method brings to more attention and interesting and has been studied and applied more and more in recent years. At present, this technology is mainly used to the study of biological macromolecules such as nucleic acids and proteins and inorganic ions and some Pharmaceuticals . This thesis has studied the application of the RRS and RNLS methods for determining some alkaloids such as berberine hydrochloride, quinine sulphate and water-soluble polymers such as polyacrylamide (PAM), sodium carboxymethyl cellulose (NaCMC) and Triton X-100. The systems of this thesis are isopoly acid (isopoly-tungstic acid and isopoly-molybdic acid) and quinine sulphate as well as berberine hydrochloride systems, polyacrylamide and four kinds of basic dyes (basic diphenyl naphthylmethane dyes, basic triphenylmethane dye, basic phenothiazine dyes and basic phenozine dyes) systems as well as PAM and one kind of acid dye (halogenated fluorescin dyes) system, NaCMC and two basic dyes (ethyl violet and nile blue sulphate) systems and Triton X-100 and halogenated fluorescin dyes system. Their RRS, SOS and FDS spectral characteristics, optimum reaction conditions, influencing factors and analytical applications are studied. The reaction mechanism, the reasons of RRS enhancement and the relationship between RRS spectra and absorption spectra are discussed.The main contents and some conclusions of the thesis are as follows:1. Isopoly Acid- Some Alkaloids Systems1.1 Resonance Rayleigh Scattering and Frequency Double Scattering, Second Order Scattering Study of Interaction of Isopoly Acid with quinine sulphateTungstic acid and molybdic acid will aggregate to form isopoly-tungstic acid and isopoly-molybdic acid, which react with quinine cation to form ion-association complexes resulting in the enhancement of RRS, SOS and FDS. The maximum peaks of RRS, SOS and FDS of isopoly-tungstic acid-quinine sulphate system are located at 470nm, 790nm and 395nm, and the detect limits are 7.7ng/ml, 8.6ng/ml and 10.9ng/ml, respectively. The maximum peaks of isopoly-molybdic acid-quinine sulphate system are located at 470nm, 940nm and 470nm, and the detect limits are 15.6ng/ml, 28.3ng/ml and 34.4ng/ml. The suitable reaction conditions and affecting factors are investigated. The RRS method of the iospoly-tungstic acid-quinine sulphate system is the most sensitive and is applied to the determination of quinine in synthetic samples and Tonic Water with satisfactory results.1.2 Isopoly acid-berberine hydrochloride systemsIsopoly-tungstic acid and isopoly-molybdic acid can also react with berberine hydrochloride to form ion-association complexes, which result in the enhancement of RRS, SOS and FDS and the appearance of new spectra. For isopoly-tungstic acid-berberine system, there are two RRS peaks located at 288nm and 369nm with similar intensities. The maximum SOS and FDS peaks are located at 540nm and 390nm, respectively . They all have high sensitivities and the detect limits are between 7.6-36.8 ng/ml. For isopoly-molybdic acid-berberine system, there is only a RRS peak located at 373nm, and the detect limit is 8.9 ng/ml. The reaction mechanism and the reasons of scattering enhancement are investigated. The RRS method has been applied to the determination of berberine in tablets and goldthread extracts with satisfactory results.2. PAM-Basic Dyes Systems 2.1 PAM-Basic phenozine DyesThe RRS method has been applied to the study of the interaction of PAM with phenozine Dyes such as neutral red (NR), safranine T (ST) and phenosafranine (PS). The maximum RRS peaks are located at 325nm (NR), 312nm (PS) and 318nm (ST), and the detect limits are 12.6ng/mK 30.0ng/ml and 28.4ng/ml, respectively. The optimum reaction conditions, the re...
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