Separation Of Chlorogenic Acid From Honeysuckle And Its Development Of Functional Product

In this paper, the reflux method of ethanol with acidity and the technique of separation with macro resin were used to extract and separate chlorogenic acid (CHA)from Flos Lonicerae. The optimal condition of reflux extraction and the parameters of adsorption were thoroughly studied.By orthogonal design, the optimal condition of extraction is: the ethanol concentration is 70%, the ethanol acidity (in terms of pH) is 4, the reflux time is 2 h. In this condition, the final yield of CHA is 5.32%; the content of CHA is 36.13% in the production. The condition of adsorption with active carbon is: the addition amount of active carbon is 1.25 g per 100 mL solution, the adsorption temperature is 60 癈, the adsorption time is 30 min, the acidity of adsorption solution (in terms of pH) is 3. In this condition, the color of adsorption solution was decreased 85% above. By comparison of eight micro adsorption, A resin with high adsorption rate and eluent rate was used to adsorb chlorogenic acid from extraction solution. The adsorption parameters are: the content of chlorogenic acid is 0.204 mg per mL adsorption solution, the acidity of adsorption solution (in terms of pH) is 4, the adsorption velocity of flow is 3 mL per min, the eluent is 30% ethanol, the eluting speed is 1.5 mL per min and the eluents volume is 4 times of resin volume. In the optimal adsorption condition, the final yield of CHA is 5.06%. the content of chlorogenic acid is 78.26% in dry productin. Several Chinese herbal medicine were selected to combine aerosol compound, such as Honeysuckle, Forsythia suspense Vahl, Glycyrrhiza uralensis, Platycodon grandi florum and Ophiopogon japonicus. The anti-bacterial and anti-oxidation tests were done to testify the effect of aerosols compound. By in vitro functional experiment, CHA has obvious effect of grownth inhibition on staphylococcus aureus and a cleaning DPPH free radical. Aerosols compound has significant in vitro effect of grownth inhibition on streptococcus pneumonriae^ staphylococcus aureus, a -hemdytic streptoccus etc.