New Application Of N-Bromosuccinimide And Permanganate Chemiluminescence System In Pharmaceutical Analysis

Application of N-bromosuccinimide and permanganate chemiluminescence system in pharmaceutical analysis is described in this paper. Two chapters arc contained in this paper.Chapter 1 describes the application of N-bromosuccinimide (NBS) chemiluminescence system, and five sections are contained in this chapter.The first section reviews the development of N-bromosuccinimide CL system. Application and mechanism research of this system arc described in detail in this section.The second section recommends a flow injection CL analysis method for the determination of 2-aminobutyric acid. The method is based on the CL emitted during the oxidation of 2-aminobutyric acid by N-bromosuccinimide in alkaline medium in the presence of fluorescent compound as energy-transfer. The detection limit is 8.0 X10-8 g/mL(3 o ), the linear range of determination is 3.0 X 10-7 - 1.0 X 10-4 g/mL with the relative standard deviation of 2.0 %(C=5 X 10-6g/mL, n=11). The recommended method has been successfully used for analysis of caries removel system with a satisfactory result.The third section describes a CL method for the determination of salbutamol. It is based on the strong inhibition of salbutamol on the CL of the reaction of NBS and fluorescein in alkaline medium. Various factors affecting the CL emission are studied in detail. The possible mechanism is also discussed. The decrease of CL emission is linear with salbutamol in the range of 8.0X 10-8~1.0X 10-5g/mL. The detection limit is 2.6X 10-8g/mL and the RSD is 3.0 %(C=3.0X 10-7g/mL, n=11). It has been successfully used for the determination of salbutamol in pharmaceutical preparation.The fourth section describes a flow-injection system for automated dissolution testing of gentamycin tablets with CL detection. A simple and sensitive flow-injection CL system forautomated dissolution testing is described and evaluated for monitoring of dissolution profiles of gentamycin tablets. The dissolution profiles of gcntamycin fast-release tablets was determined, which demonstrates the stability, great sensitivity, large dynamic measuring range and robustness of the system.The fifth section presents a novel and sensitive CL method for the determination of aminomethylbenzoic acid and aminophylline coupled with flow-injection analysis technique. It is based on the inhibition effect of the studied drugs on the CL emission of NBS-luminol system. Under the optimum conditions, the decreased CL intensity is linear with the concentration of aminomethylbenzoic acid in the range of 2.0x10-8 to 1.0xl0-6gml-1 and with the concentration of aminophylline in the range of 1.0x10-7 to 7.0xl0-6gml-1. respectively. The detection limit is 7.0xl0-9gml-1 for aminomethylbenzoic acid (3o) and 3.4x10-8gml-1 for aminophylline (3o). The relative standard deviations for 11 parallel measurements of 2.0x10-7gml-1 aminomethylbenzoic acid and 1.0x10-6gml-1 aminophylline are 2.6 and 3.0 %, respectively. The proposed methods have been applied for the determination of the studied drugs in their pharmaceutical formulations with satisfactory results.Chapter 2 describes the application of permanganate chemiluminescence system, and three sections are contained in this chapter.The first section reviewed the development of acidic permanganate CL system since 1994.The second section recommends a sensitive flow injection CL method for the determination of indomethacin. It is based on CL emitted during the oxidation of indomethacin by KMnO4 in polyphosphoric acid medium. CL can be greatly enhanced in the presence of formaldehyde. The method permits the determination of indomethacin over a concentration range of 2.0x10-9~1.0x10-6 g/mL with a RSD of 2.3 % for the determination of 1.0x10-8 g/mL indomethacin (n = 11). The recommended method is suitable for automatic and continues analysis. The recommended method has been successfully used for analysis of commercial formulations without any sample pre-treatment.The third section proposes a flow injection analysis method for the determination of carbazochrom...