Study On Medicinal Macroporous Adsorption Resins And Their Application On Seperation And Purification Of Traditional Chinese Medicine

Macroporous adsorption resin(MAR) is an adsorptive polymer with macroreticular structure. In recent years, MAR has been used in the seperation and purification of TCM and natural products more broadly for its special adsorption performance to water soluble materials. Now, Some organic residues still remain in most of the commercial MARs produced by domestic factories, which induces the unsafely of the drugs performed by MARs. In order to build a simple analytical method of the residues of MARs and effective elution method of MARs, we performed the following experiments.Organic residues in nine kinds MARs were identified by GC-MS. Toluene, diethylbenzene and naphthalene existed in all MARs. The other benzene derivatives, such as xylene, strylene, divinylbenzene and other residues were also identified, but their quantities were little. Dozens of alkanes were also detected. Among them, the quantities of decane, undecane and dodecane were very high.During studied the pretreatment method of MAR detected by GC and UV, we found that solvent and tempreture were two key elements in this procedure. Under a certain tempreture, MAR was almost clean after it was eluted by 5% NaOH for 2 bed volume of MAR(BV) and then by organic solvent for only 3~5BV. The operation is easy, short time and had little contamination to the environment. The amount of solvent used in the pretreatment were little. Besides, We found that when the absorbance value was 0.2~0.4( λ =254nm), the organic residues were also very limited detected by GC. That was the absorbance value could represent the amount of the organic residues in some extent and we could use the UV method to detect the organic residues of MARs conveniently.A method was established to detect the organic residues in MARs and medicines with GC method. The detection was easy and sensitive. All the ten organic residues could be determined through a column simultaneously in a short time.A standard was also made for medicinal MARs: in the dried MARs, benzene shouldn't be more than 2ppm; toluene, xylene, strylene, diethylbenzene, divinylbenzene, naphthalene, decane, undecane and dodecane should't be more than 20ppm.We also studied the application of MARs in the seperation and purification of TCM. The main active components of Gardenia jasminoides Ellis, and Hypericum perforatum L.were gardenoside, crocins and flavonoids. But studies on the seperation and purification of active components in both plants by MARs had not been reported. We studied the adsorption performance of 13 MARs to irodoids in Gardenia jasminoides Ellis. Among them, HPD-100,AB-8, SP825 and SP207 had a bigger adsorption capacities. Resin AB-8 had a good seperation performance. At the same time, we established a method of seperation and purification of the yellow pigment and the total glycosides in Gardenia jasminoides Ellis by resin AB-8. We also studied adsortion performance of 9 resins to flavonoids in Hypericum perforatum L. Among them, resin HPD-500, HPD100, AB-8 and NKA-9 had a bigger adsorption capacities.Advance in the types, properties, principle of adsorption of MARs and their application in the medical and pharmerceutical industry were summerized, especially the research and development of MARs on the seperation and purification of the active components in TCM and natural products were emphasized.