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Clear Jets Of Particles In Sea Buckthorn Extract And Purify Process

Posted on:2008-01-25Degree:MasterType:Thesis
Country:ChinaCandidate:W W PuFull Text:PDF
GTID:2191360212485604Subject:Ecology
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"Chinese herbal medicine healthy food" rests on Chinese medicine theory for the health keeping need; it carries on the special processing to the traditional Chinese medicine so that the health-care effect of the traditional Chinese medicine will be more remarkable. In this thesis we studies the extraction craft and the purifying technology thoroughly to provide the basis of healthy food production which not only can reduce the dose of the prescription effectively, but also can keep its efficiency and advantageous.Our research work take the Flos Lonicerae, Herba Menthae, Fructus Arctii, Fructus Hippophaes and other Chinese medicine as samples. Base on the evaluation index and method, we conduct a systematic research on extract condition and purifying technology. Through such research, we seek a way to improve the Chinese medicine healthy food's quality and promote healthy food's development.To establish the index and method is the key in evaluating the rationality in preparation and craft of Chinese traditional compound medicine, and it is also an important aspect in estimating the quality of the process. According to the efficacy of Qing Hou Ke Li and the characters of each component in the prescription, We select three of the effective component - Chlorogenic acid, Arctiin and total flavonoids asthe index of the craft. To choose the best preparation process, high performance liquid chromatography (HPLC) and the ultraviolet radiation spectral analysis are used to determine the content of the effective components accurately.The effective component of the pack of Chinese traditional medicine fully lixiviating is the precondition to pledge the efficacy of this healthy foods. Ascertaining the course of distill the medicine, the best extraction process of volatile oil and Chinese traditional compound medicine have been chosen through one-factor analysis and the orthogonal experiments: (1) the optimum extraction process of Herba Menthae volatile oil: 10 times of water and distillation for 8h. (2) the optimum extraction process of Qing Hou Ke Li: 70% ethanol 10 times of raw material to soak for 2 hours, and then 1.5 hours' distillation for 3 times.The purificatory craft is useful to solve the problems of the common product which are "thick, mass, black" and it also is the crutial approach to guarantee the quality of the Chinese traditional medicine. Through investigating the reserved rate and the variety of content of effective component, we systematically study methods of eliminating impurity (ultracentrifugation technology, ultrafilter technology, macroporous adsorption resins) and analyse the probems which appearing in these kinds of technique which have been applied to eliminate impurities of the medicament. The analysis can be used for reference in the process ofpurifications for other Chinese traditional medicine.The conclusions of the experiments indicate that: through the methods of ultracentrifugation technology, ultrafilter technology, the reserved rate is very high and the eliminated rate of the impurities is very low. So the two methods of eliminating impurity lead to the low content of the effective components and they will not reduce dose. Though the method of macroporous adsorption resins, the effective components can be riched and the impurities can be reduced. The content of chlorogenic acid, Actiin and total flavonoids in the medicament which has been dealed with by the method of macroporous adsorption resins can be enhanced remarkably.
Keywords/Search Tags:Qing Hou Ke Li, chlorogenic acid, total flavonoids, Actiin, ultracentrifugation, ultrafilter, macroporous adsorption resins
PDF Full Text Request
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